| 1. |
- Wei, Jian-Feng, et al.
(författare)
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Formation of Kv2.1-FAK complex as a mechanism of FAK activation, cell polarization and enhanced motility
- 2008
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Ingår i: Journal of Cellular Physiology. - : Wiley. - 0021-9541 .- 1097-4652. ; 217:2, s. 544-557
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Tidskriftsartikel (refereegranskat)abstract
- Focal adhesion kinase (FAK) plays key roles in cell adhesion and migration. We now report that the delayed rectifier Kv2.1 potassium channel, through its LD-like motif in N-terminus, may interact with FAK and enhance phosphorylation of FAK(397) and FAK(576/577) Overlapping distribution of Kv2.1 and FAK was observed on soma and proximal dendrites of cortical neurons. FAK expression promotes a polarized membrane distribution of the Kv2.1 channel. In Kv2.1-transfected CHO cells, formation of the Kv2.1-FAK complex was stimulated by fibronectin/integrin and inhibited by the K channel blocker tetraethylammonium (TEA). FAK phosphorylation was minimized by shRNA knockdown of the Kv2.1 channel, point mutations of the N-terminus, and TEA, respectively. Cell migration morphology was altered by Kv2.1 knockdown or TEA, hindering cell migration activity. In wound healing tests in vitro and a traumatic injury animal model, Kv2.1 expression and co-localization of Kv2.1 and FAK significantly enhanced directional cell migration and wound closure. It is suggested that the Kv2.1 channel may function as a promoting signal for FAK activation and cell motility.
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| 2. |
- Gao, Xin-Fen, et al.
(författare)
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Three new species of Corydalis (Fumariaceae) from northwestern Sichuan China
- 2008
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Ingår i: Novon. - : Missouri Botanical Garden Press. - 1055-3177 .- 1945-6174. ; 18:3, s. 330-335
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Tidskriftsartikel (refereegranskat)abstract
- The jiuding Ridge (Sichuan, China) has been explored during the past several years by a team from chengdu Institute of Biology. Chinese Academy of Sciences, and three undescribed species of Corydalis DC. (Fumariaceae) have been revealed and are apparently endemic to this mountain. The new species were observed in forest (C. capitata X.F. Cao, Liden & Y.W. Wang), and alpine rocky limestone areas (C. schistostigma X.F. Cao, Liden, Y.W. Wang & Y.L. Peng). The two first species have their closest relatives in the Wolong-Balangshan District in the Wolong-Balangshan District (Wenchuan. Sichuan). Corydalis aeaeae differs from C. panada Liden & Y.W. Wang in its small size, few-flowered racemes, and broadly obtuse outer petals with low, short erests. Corydalis capitata differs from the C. flexuosa Franchet complex in the capitate racemes, small flowers with deeply serrate petals, and the peculiar, thin, strictly ecect lateral branches. corydalis schistostinma is unique in the C. curviflora Maximowicz ex Hemsley group, in the deeply cleft stigma and very forward-projecting crests to the inner petals, and possibly has iits affinities more to the north and northwest in the Hengduan Mountains.
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| 3. |
- Guo, Xin, et al.
(författare)
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Direct Coupling of Plasmonic and Photonic Nanowires for Hybrid Nanophotonic Components and Circuits
- 2009
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Ingår i: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 9:12, s. 4515-4519
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Tidskriftsartikel (refereegranskat)abstract
- We report direct coupling of plasmonic and photonic nanowires using ultracompact near-field interaction. Photon-plasmon coupling efficiency up to 80% with coupling length down to the 200 nm level is achieved between individual Ag and ZnO nanowires. Hybrid nanophotonic components, including polarization splitters, Mach-Zehnder interferometers, and microring cavities, are fabricated out of coupled Ag and ZnO nanowires. These components offer relatively low loss with subwavelength confinement; a hybrid nanowire microcavity exhibits a Q-factor of 520.
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| 4. |
- Larsson, Lars, 1952-, et al.
(författare)
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Adaptation by alternative RNA splicing of slow troponin T isoforms in type 1 but not type 2 Charcot-Marie-Tooth disease
- 2008
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Ingår i: American Journal of Physiology - Cell Physiology. - : American Physiological Society. - 0363-6143 .- 1522-1563. ; 295:3, s. 722-731
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Tidskriftsartikel (refereegranskat)abstract
- Slow troponin T (TnT) plays an indispensable role in skeletal muscle function. Alternative RNA splicing in the NH2-terminal region produces high-molecular-weight (HMW) and low-molecular-weight (LMW) isoforms of slow TnT. Normal adult slow muscle fibers express mainly HMW slow TnT. Charcot-Marie-Tooth disease (CMT) is a group of inherited peripheral polyneuropathies caused by various neuronal defects. We found in the present study that LMW slow TnT was significantly upregulated in demyelination form type 1 CMT (CMT1) but not axonal form type 2 CMT (CMT2) muscles. Contractility analysis showed an increased specific force in single fibers isolated from CMT1 but not CMT2 muscles compared with control muscles. However, an in vitro motility assay showed normal velocity of the myosin motor isolated from CMT1 and CMT2 muscle biopsies, consistent with their unchanged myosin isoform contents. Supporting a role of slow TnT isoform regulation in contractility change, LMW and HMW slow TnT isoforms showed differences in the molecular conformation in conserved central and COOH-terminal regions with changed binding affinity for troponin I and tropomyosin. In addition to providing a biochemical marker for the differential diagnosis of CMT, the upregulation of LMW slow TnT isoforms under the distinct pathophysiology of CMT1 demonstrates an adaptation of muscle function to neurological disorders by alternative splicing modification of myofilament proteins.
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| 5. |
- Li, Li, et al.
(författare)
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Effects of 2-methoxyestradiol on endometrial carcinoma xenografts
- 2007
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Ingår i: Journal of Cancer Research and Clinical Oncology. - : Springer Science and Business Media LLC. - 0171-5216 .- 1432-1335. ; 133:5, s. 315-320
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: We have previously demonstrated that 2-methoxyestradiol (2-ME) inhibits the growth of human endometrial cancer HEC-1-A and RL-95-2 cells in vitro. In this study, we examined the effects of 2-ME on human endometrial carcinoma in severe combined immune deficient (SCID) mice. The potential side effects of 2-ME on SCID mice were also investigated. Methods: Severe combined immune deficient mice were injected with HEC-1-A cells (1 × 10 6/mouse) and a 18 day administration of 2-ME was followed after 1 week cell implantation. Tumor volume, weight, body weight and blood chemistry were determined. Tumor tissues were examined with an antibody against the proliferative cell nuclear antigen (PCNA) and Ki-67. Liver, spleen, kidney, heart, lung and uterus were screened by pathological examinations. Results: 2-ME (100 mg/kg p.o.) did not inhibit the growth of human endometrial carcinoma as compared to control. Necrotic areas were similar in both 2-ME-treated and -untreated tumor tissues. The expressions of PCNA and Ki-67 were similar in 2-ME-treated and untreated tumor sections. The wet weight of uterus was increased to more than threefold. The epithelial cells and glands in endometrium were increased. No significant difference was detected in blood AST, ALT and BUN. Conclusions: 2-ME has no antitumor effects on human endometrial carcinoma in our animal model. Its proliferative effects on endometrium and uterus might limit its use in gynecological cancers.
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| 6. |
- Yang, Tao, et al.
(författare)
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A spatial assessment of hydrologic alteration caused by dam construction in the middle and lower Yellow River, China
- 2008
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Ingår i: Hydrological Processes. - : Wiley. - 0885-6087 .- 1099-1085. ; 22:18, s. 3829-3843
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Tidskriftsartikel (refereegranskat)abstract
- The ‘range of variability approach’ (RVA) and mapping technique are used to investigate the spatial variability of hydrologic alterations (HA) due to dam construction along the middle and lower Yellow River, China, over the past live decades. The impacts of climate variability oil hydrological process have been removed during wet and dry periods and the focus IS Oil the impacts of human activities, Such as dam construction, on hydrological processes. Results indicate the following: (1) The impacts of the Sanmenxia reservoir oil the hydrologic alteration are relatively slight with a mean HA value of 0.48, ranking in the last place among the four large reservoirs. (2) Xiaolangdi reservoir has significantly changed the natural flow regime downstream with mean HA value of 0.56, ranking it in first place among the large reservoirs. (3) The results of ranked median degrees of 33 hydrologic alteration indicators for 10 stations in the Yellow River show that the hydrologic alteration of Huayuankou ranks the highest among 10 stream gauges. (4) Impacts of reservoirs on hydrological processes downstream of the dams are closely associated with the regulating activities of the reservoirs. At the same time, alterations of streamflow regimes resulting from climatic changes (e.g. precipitation variability) make the situation more complicated and more hydrological observations will be necessary for further analysis. The results of the current study will be greatly beneficial to the regional water resources management and restoration of eco-environmental systems in the middle and lower Yellow River characterized by intensified dam construction under a changing environment. Copyright (C) 2008 John Wiley & Sons, Ltd.
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| 7. |
- Zhang, Jin-Ting, et al.
(författare)
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Nuclear to cytoplasmic shift of p33ING1b protein from normal oral mucosa to oral squamous cell carcinoma in relation to clinicopathological variables
- 2008
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Ingår i: Journal of Cancer Research and Clinical Oncology. - : Springer Science and Business Media LLC. - 0171-5216 .- 1432-1335. ; 134:3, s. 421-426
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: p33ING1b, as a candidate tumour suppressor gene, has been found to be expressed a proportion of oral squamous cell carcinomas (OSCCs), however, its clinicopathological significance is not studied yet. Our aim was to investigate association of p33ING1b expression with clinicopathological variables and particularly interesting new cysteine-histidine rich protein (PINCH) in OSCCs. Methods: p33ING1b expression was immumohistochemically examined in 20 normal oral mucosa specimens and 49 OSCCs. Results: Normal squamous cells showed only p33ING1b nuclear expression (no cytoplasmic expression), with a rate of 90% positive cases. While 24% of OSCCs appeared cytoplasmic expression (11 of them with weak nuclear staining) and the rest tumours (76%) were negative for p33 ING1b. Furthermore, the cases having lymph node metastasis showed a higher frequency of positive cytoplasmic expression than those without metastasis (P = 0.03). The p33ING1b cytoplasmic expression was positively related to PINCH expression (P = 0.04), the cases positive for both proteins had a high rate of the metastasis (P = 0.03). Conclusions: The transfer of p33ING1b protein from the nucleus to the cytoplasm may result in loss of normal cellular function of the protein, which might play a role in the tumourigenesis and metastasis of OSCCs. © 2007 Springer-Verlag.
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