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Increase of faecal ...
Increase of faecal tryptic activity relates to changes in the intestinal microbiome : analysis of Crohn's disease with a multidisciplinary platform.
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- Midtvedt, Tore (författare)
- Karolinska Institutet
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- Zabarovsky, Eugene (författare)
- Karolinska Institutet, Stockholm, Sweden
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- Norin, Elisabeth (författare)
- Karolinska Institutet
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- Bark, Johan (författare)
- Stockholm Gastro Center, Sophiahemmet Hospital, Stockholm, Sweden
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- Gizatullin, Rinat (författare)
- Karolinska Institutet, Stockholm, Sweden
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- Kashuba, Vladimir (författare)
- Karolinska Institutet
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- Ljungqvist, Olle, 1954- (författare)
- Karolinska Institutet,Örebro universitet,Institutionen för hälsovetenskap och medicin,Region Örebro län
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- Zabarovska, Veronika (författare)
- Karolinska Institutet, Stockholm, Sweden
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- Möllby, Roland (författare)
- Karolinska Institutet
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- Ernberg, Ingemar (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2013-06-20
- 2013
- Engelska.
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:6, s. e66074-
- Relaterad länk:
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https://doi.org/10.1...
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- OBJECTIVE: To investigate-by molecular, classical and functional methods-the microbiota in biopsies and faeces from patients with active Crohn's disease (CD) and controls.DESIGN: The microbiota in biopsies was investigated utilizing a novel molecular method and classical cultivation technology. Faecal samples were investigated by classical technology and four functional methods, reflecting alterations in short chain fatty acids pattern, conversion of cholesterol and bilirubin and inactivation of trypsin.RESULTS: By molecular methods we found more than 92% similarity in the microbiota on the biopsies from the two groups. However, 4.6% of microbes found in controls were lacking in CD patients. Furthermore, NotI representation libraries demonstrate two different clusters representing CD patients and controls, respectively. Utilizing conventional technology, Bacteroides (alt. Parabacteroides) was less frequently detected in the biopsies from CD patients than from controls. A similar reduction in the number of Bacteroides was found in faecal samples. Bacteroides is the only group of bacteria known to be able to inactivate pancreatic trypsin. Faecal tryptic activity was high in CD patients, and inversely correlated to the levels of Bacteroides.CONCLUSIONS: CD patients have compositional and functional alterations in their intestinal microbiota, in line with the global description hypothesis rather than the candidate microorganism theory. The most striking functional difference was high amount of faecal tryptic activity in CD patients, inversely correlated to the levels of Bacteroides in faeces.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
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- art (ämneskategori)
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Midtvedt, Tore
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Zabarovsky, Euge ...
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Norin, Elisabeth
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Bark, Johan
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Gizatullin, Rina ...
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Kashuba, Vladimi ...
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Ljungqvist, Olle ...
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Zabarovska, Vero ...
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Möllby, Roland
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Ernberg, Ingemar
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- MEDICIN OCH HÄLSOVETENSKAP
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Örebro universitet
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Karolinska Institutet