| 1. |
- Dong, Yihui, et al.
(författare)
-
Excellent Trace Detection of Proteins on TiO2Nanotube Substrates through Novel Topography Optimization
- 2020
-
Ingår i: The Journal of Physical Chemistry C. - : American Chemical Society (ACS). - 1932-7447 .- 1932-7455. ; 124:50, s. 27790-27800
-
Tidskriftsartikel (refereegranskat)abstract
- For improving the surface-enhanced Raman scattering (SERS) performance of nanomaterials to achieve trace detection of proteins and complex biological systems, structural and topographical control is one of the important strategies. In this work, a facial and effective method to optimize the topography of TiO2 nanotube arrays (TNAs) is demonstrated, together with a significant enhancement of the SERS performance of cytochrome C (Cyt C) on TNAs. An enhancement factor (EF) up to 104, which is obtained with the newly developed method on the basis of the quantification of atomic force microscopy (AFM)-measured interaction force, is achieved, corresponding to a superior detection limit of Cyt C down to 10-7 M. The main reason is that adjusting the fluoride contents in an electrolyte (from 0.4 to 0.1 wt %) can reduce the content and sizes of cracks, as well as the tube ruptures of TNAs, where the fluoride content at 0.2 wt % can successfully provide the excellent and optimized topography of TNAs. The TNAs with the optimized topography, especially those with larger tube diameters, demonstrated the importance of structural integrity on a remarkably excellent SERS performance in the trace detection of proteins. The proposed method will stimulate the development and optimization of the active substrate on the SERS applications in biology, bioanalysis, and nanoscience. © 2020 American Chemical Society.
|
|
| 2. |
- Shi, Chen, et al.
(författare)
-
The Potential of Low Molecular Weight Heparin to Mitigate Cytokine Storm in Severe COVID-19 Patients : A Retrospective Cohort Study
- 2020
-
Ingår i: Clinical and Translational Science. - : WILEY. - 1752-8054 .- 1752-8062. ; 13:6, s. 1087-1095
-
Tidskriftsartikel (refereegranskat)abstract
- On March 11, 2020, the World Health Organization declared its assessment of coronavirus disease 2019 (COVID-19) as a global pandemic. However, specific anti-severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) drugs are still under development, and patients are managed by multiple complementary treatments. We performed a retrospective analysis to compare and evaluate the effect of low molecular weight heparin (LMWH) treatment on disease progression. For this purpose, the clinical records and laboratory indicators were extracted from electronic medical records of 42 patients with COVID-19 (21 of whom were treated with LMWH, and 21 without LMWH) hospitalized (Union Hospital of Huazhong University of Science and Technology) from February 1 to March 15, 2020. Changes in the percentage of lymphocytes before and after LMWH treatment were significantly different from those in the control group (P = 0.011). Likewise, changes in the levels of D-dimer and fibrinogen degradation products in the LMWH group before and after treatment were significantly different from those in the control group (P = 0.035). Remarkably, IL-6 levels were significantly reduced after LMWH treatment (P = 0.006), indicating that, besides other beneficial properties, LMWH may exert an anti-inflammatory effect and attenuate in part the "cytokine storm" induced by the virus. Our results support the use of LMWH as a potential therapeutic drug for the treatment of COVID-19, paving the way for a subsequent well-controlled clinical study.
|
|
