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- Andersson, K, et al.
(författare)
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Effect of a-fluoromethylhistidine-evoked histamine depletion on ultrastructure of endocrine cells in acid-producing mucosa of stomach in mouse, rat and hamster
- 1996
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Ingår i: Cell and Tissue Research. - 1432-0878. ; 286:3, s. 84-375
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Tidskriftsartikel (refereegranskat)abstract
- The oxyntic mucosa of the mammalian stomach is rich in endocrine cells, such as ECL cells, A-like cells, somatostatin cells, D1/P cells and, in some species, enterochromaffin cells. The various endocrine cell types can be distinguished on the basis of their characteristic cytoplasmic granules and vesicles. The ECL cells contain numerous large secretory vesicles and relatively few, small electron-dense granules and small clear microvesicles. We have suggested that in the rat the ECL cells contain most of the gastric histamine with the secretory vesicles as the major histamine storage site in these cells. alpha-Fluoromethylhistidine is an irreversible inhibitor of histidine decarboxylase, the histamine-forming enzyme. We have previously shown that this enzyme inhibitor depletes histamine from the ECL cells in the rat and reduces the number of secretory vesicles in the cytoplasm. In the present study, we have examined whether alpha-fluoromethylhistidine affects the ECL cells in other species and whether it affects other types of endocrine cells in the oxyntic mucosa of the rat. Mice, rats and hamsters were treated with the inhibitor (3 mg/kg per h) via minipumps subcutaneously for 24 h. This treatment lowered the oxyntic mucosal histamine concentration by 65-90% and the number and volume density of the secretory vesicles by 85-95% in the ECL cells of the three species examined. In contrast, the number and volume density of granules and microvesicles were not greatly affected. No evidence was found for an effect of alpha-fluoromethylhistidine on A-like cells, somatostatin cells or D1/P cells of the rat stomach, suggesting that, unlike the ECL cells, they do not contain histamine.
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| 7. |
- Yuan, L, et al.
(författare)
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The synaptonemal complex protein SCP3 can form multistranded, cross-striated fibers in vivo
- 1998
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Ingår i: The Journal of cell biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 142:2, s. 331-339
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Tidskriftsartikel (refereegranskat)abstract
- The synaptonemal complex protein SCP3 is part of the lateral element of the synaptonemal complex, a meiosis-specific protein structure essential for synapsis of homologous chromosomes. We have investigated the fiber-forming properties of SCP3 to elucidate its role in the synaptonemal complex. By synthesis of SCP3 in cultured somatic cells, it has been shown that SCP3 can self-assemble into thick fibers and that this process requires the COOH-terminal coiled coil domain of SCP3, as well as the NH2-terminal nonhelical domain. We have further analyzed the thick SCP3 fibers by transmission electron microscopy and immunoelectron microscopy. We found that the fibers display a transversal striation with a periodicity of ∼20 nm and consist of a large number of closely associated, thin fibers, 5–10 nm in diameter. These features suggest that the SCP3 fibers are structurally related to intermediate filaments. It is known that in some species the lateral elements of the synaptonemal complex show a highly ordered striated structure resembling that of the SCP3 fibers. We propose that SCP3 fibers constitute the core of the lateral elements of the synaptonemal complex and function as a molecular framework to which other proteins attach, regulating DNA binding to the chromatid axis, sister chromatid cohesion, synapsis, and recombination.
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| 8. |
- Zeng, J, et al.
(författare)
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Are neuronal markers and neocortical graft-host interface influenced by housing conditions in rats with cortical infarct cavity?
- 1999
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Ingår i: Brain Research Bulletin. - 0361-9230. ; 48:2, s. 165-171
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Tidskriftsartikel (refereegranskat)abstract
- The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of the infarct cavity. In 8 of 18 transplants, 4 in each experimental group, part of the transplants protruded through the thin glial membrane that delineated the transplant-host interface into the adjacent host brain tissue. Microtubule associated protein 2 immunostained sections indicated bridging of dendrites in the host-transplant interface. Synaptophysin immunoreactivity was significantly higher in grafts than in contralateral cortex. However, graft morphology and neuronal marker immunoreactivity did not differ between rats housed in standard and activity stimulating cages.
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| 10. |
- Zhu, J. W., et al.
(författare)
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Reductase specificity and the ratio regulation of E/Z isomers in pheromone biosynthesis of the European corn borer, Ostrinia nubilalis (Lepidoptera Pyralidae)
- 1996
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Ingår i: Insect Biochemistry and Molecular Biology. - : Elsevier BV. - 0965-1748. ; 26:2, s. 171-176
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Tidskriftsartikel (refereegranskat)abstract
- Species specificity of moth sex pheromones is in many cases achieved by means of specific blends rather than by specific components. Two pheromone strains of the European corn borer, Ostrinia nubilalis, use (E)- and (Z)-11-tetradecenyl acetate in different ratios as their pheromone, but show the same ratio of the pheromone precursors (70:30 E/Z-11-tetradecenoic acid). The hypothesis that the ratio of the pheromone components in the two strains and their hybrids is controlled by the specificity of the reductase system, responsible for conversion of acid to the corresponding alcohol precursors, was tested. Deuterium-labeled alcohols, aldehydes and fatty acids corresponding to the two pheromone components were topically applied to the pheromone glands in different ratios and their selective incorporation into pheromone components was determined by gas chromatography with mass selective detection. Acetylation of the (E)- and (Z)-11-tetradecenols was unselective, whereas the corresponding aldehydes and acids were selectively incorporated into the pheromone components. Z strain females selectively metabolized the Z-isomers whereas E strain females converted the E-isomers. The E strain being the most selective of the two strains. Hybrids converted both geometric isomers. The relative conversion rate of both E- and Z-isomers of all tetradecenoic acids with the double bond in positions from 7-12, was also determined. In addition to the Δ11-isomers, the E strain females converted (E)-8-tetradecenoic acid into acetate and the Z strain females converted (E)-12-tetradecenoic acid. None of these substrates occur naturally in the pheromone gland, but (E)-12-tetradecenyl acetate is a pheromone component of the Asian corn borer O. furnacalis. Thus the possibility for conversion of (E)-12-tetradecenoic acid to acetate in the Z strain, as well as the earlier reported conversion of (Z)-11-tetradecenoic acid to acetate in O. furnacalis, suggests that O. furnacalis is closest related to the Z strain of O. nubilalis.
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