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- Sun, X, et al.
(författare)
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The hrp23 protein in the balbiani ring pre-mRNP particles is released just before or at the binding of the particles to the nuclear pore complex
- 1998
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Ingår i: The Journal of cell biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 142:5, s. 1181-1193
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Tidskriftsartikel (refereegranskat)abstract
- Balbiani ring (BR) pre-mRNP particles reside in the nuclei of salivary glands of the dipteran Chironomus tentans and carry the message for giant-sized salivary proteins. In the present study, we identify and characterize a new protein component in the BR ribonucleoprotein (RNP) particles, designated hrp23. The protein with a molecular mass of 20 kD has a single RNA-binding domain and a glycine-arginine-serine–rich auxiliary domain. As shown by immunoelectron microscopy, the hrp23 protein is added to the BR transcript concomitant with transcription, is still present in the BR particles in the nucleoplasm, but is absent from the BR particles that are bound to the nuclear pore complex or are translocating through the central channel of the complex. Thus, hrp23 is released just before or at the binding of the particles to the nuclear pore complex. It is noted that hrp23 behaves differently from two other BR RNP proteins earlier studied: hrp36 and hrp45. These proteins both reach the nuclear pore complex, and hrp36 even accompanies the RNA into the cytoplasm. It is concluded that each BR RNA-binding protein seems to have a specific flow pattern, probably related to the particular role of the protein in gene expression.
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- Urabe, Tadahisa, et al.
(författare)
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Regeneration across a partial defect in rat sciatic nerve encased in a silicone chamber
- 1996
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Ingår i: Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery. - 0284-4311. ; 30:1, s. 7-15
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Tidskriftsartikel (refereegranskat)abstract
- Forty-four rat sciatic nerves with partial defects were repaired with a silicone chamber. Each partial defect was created by resecting a 10 mm segment from the tibial fascicle leaving the peroneal fascicle intact. The proximal and the distal stumps of the tibial fascicle together with the intact peroneal fascicle were encased in a single silicone chamber. After seven days a fibrin matrix had surrounded the peroneal fascicle and spanned the defect between the tibial stumps. This matrix was later invaded by non-neuronal cells and regenerating axons. Non-myelinated nerve fibres had almost regenerated across the defect by 16 days. The tetanic force of the gastrocnemius muscle 120 days after repair showed 80% recovery, which was no different from that of partial defects repaired with conventional nerve grafts. The results suggest that the silicone chamber technique could be applicable to the treatment of partially transected nerve trunks.
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- Zhang, Yuan, et al.
(författare)
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Pituitary adenylate cyclase activating peptide expression in the rat dorsal root ganglia : up-regulation after peripheral nerve injury
- 1996
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Ingår i: Neuroscience. - : Elsevier BV. - 0306-4522. ; 74:4, s. 110-1099
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Tidskriftsartikel (refereegranskat)abstract
- Pituitary adenylate cyclase activating peptide (PACAP) is expressed in a population of capsaicin-sensitive primary sensory neurons of small to medium size in the rat. In the present report we have examined the effect of sciatic nerve injury (unilateral transection) on PACAP expression (immunocytochemistry, radioimmunoassay, in situ hybridization and northern blot analysis) in dorsal root ganglia at the lumbar level and on immunoreactive PACAP in the spinal cord and in the sciatic nerve stump. For comparison, calcitonin gene-related peptide was examined. In dorsal root ganglia of the intact side immunoreactive PACAP and PACAP messenger RNA were localised to a population of nerve cell bodies of small to medium size. In dorsal root ganglia on the injured side, PACAP-immunoreactive nerve cell bodies were more numerous and PACAP messenger RNA was considerably more abundant as studied 14 days after sciatic nerve transection. By contrast, calcitonin gene-related peptide-containing nerve cell bodies were numerous and rich in calcitonin gene-related peptide messenger RNA in dorsal root ganglia on the intact side, while after transection both the number of immunoreactive nerve cell bodies and their content of messenger RNA were markedly reduced. There were indications of axotomy-induced expression of PACAP messenger RNA in larger neurons. In the dorsal horn of the spinal cord on the intact side PACAP and calcitonin gene-related peptide-immunoreactive fibres were densely accumulated in the superficial layers. On the transected side the densities of both PACAP and calcitonin gene-related peptide-immunoreactive nerve fibres were reduced in the medial part. The data obtained indicate a marked up-regulation of PACAP in sensory neurons following peripheral nerve injury. Since PACAP depresses a C-fibre evoked flexion reflex, this may have implications for sensory transmission. Further, in view of the known promoting effects of PACAP on neuronal survival and differentiation and non-neuronal cell growth as well as its proinflammatory effects a role of PACAP in the neuronal and periaxonal tissue restoration after injury is not inconceivable.
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