| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 6. |
- Thomas, M, et al.
(författare)
-
Combining Asian-European Genome-Wide Association Studies of Colorectal Cancer Improves Risk Prediction Across Race and Ethnicity
- 2023
-
Ingår i: medRxiv : the preprint server for health sciences. - : Cold Spring Harbor Laboratory.
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Polygenic risk scores (PRS) have great potential to guide precision colorectal cancer (CRC) prevention by identifying those at higher risk to undertake targeted screening. However, current PRS using European ancestry data have sub-optimal performance in non-European ancestry populations, limiting their utility among these populations. Towards addressing this deficiency, we expanded PRS development for CRC by incorporating Asian ancestry data (21,731 cases; 47,444 controls) into European ancestry training datasets (78,473 cases; 107,143 controls). The AUC estimates (95% CI) of PRS were 0.63(0.62-0.64), 0.59(0.57-0.61), 0.62(0.60-0.63), and 0.65(0.63-0.66) in independent datasets including 1,681-3,651 cases and 8,696-115,105 controls of Asian, Black/African American, Latinx/Hispanic, and non-Hispanic White, respectively. They were significantly better than the European-centric PRS in all four major US racial and ethnic groups (p-values<0.05). Further inclusion of non-European ancestry populations, especially Black/African American and Latinx/Hispanic, is needed to improve the risk prediction and enhance equity in applying PRS in clinical practice.
|
|
| 7. |
|
|
| 8. |
- You, Pengyao, et al.
(författare)
-
Interfacial oxidized Pd species dominate catalytic hydrogenation of polar unsaturated bonds
- 2024
-
Ingår i: Nano Reseach. - : Springer. - 1998-0124 .- 1998-0000. ; 17:1, s. 235-244
-
Tidskriftsartikel (refereegranskat)abstract
- The determination of catalytically active sites is crucial for the design of efficient and stable catalysts toward desired reactions. However, the complexity of supported noble metal catalysts has led to controversy over the locations of catalytically active sites (e.g., metal, support, and metal/support interface). Here we develop a structurally controllable catalyst system (Pd/SBA-15) to reveal the catalytic active sites for the selective hydrogenation of ketones to alcohol using acetophenone hydrogenation as model reaction. Systematic investigations demonstrated that unsupported Pd nanocrystals have no catalytic activity for acetophenone hydrogenation. However, oxidized Pd species were catalytically highly active for acetophenone hydrogenation. The catalytic activity decreased with the decreased oxidation state of Pd. This work provides insights into the hydrogenation mechanism of ketones but also other unsaturated compounds containing polar bonds, e.g., nitrobenzene, N-benzylidene-benzylamine, and carbon dioxide.
|
|
| 9. |
- Zhou, XY, et al.
(författare)
-
Mechanisms of Anergic Inflammatory Response in Nasopharyngeal Carcinoma Cells Despite Ubiquitous Constitutive NF-κB Activation
- 2022
-
Ingår i: Frontiers in cell and developmental biology. - : Frontiers Media SA. - 2296-634X. ; 10, s. 861916-
-
Tidskriftsartikel (refereegranskat)abstract
- Commensal microbes cross talk with their colonized mucosa. We show that microbes and their cell wall components induce an inflammatory response in cultured human mucosal cells derived from the nonmalignant nasopharyngeal epithelium (NNE) cells in vitro. NNE cells show significant induction of NF-κB with nuclear shuttling and inflammatory gene response when exposed to Gram-positive bacteria (streptococci) or peptidoglycan (PGN), a component of the Gram-positive bacterial cell wall. This response is abrogated in nasopharyngeal carcinoma (NPC)–derived cell lines. The inflammatory response induced by NF-κB signaling was blocked at two levels in the tumor-derived cells. We found that NF-κB was largely trapped in lipid droplets (LDs) in the cytoplasm of the NPC-derived cells, while the increased expression of lysine-specific histone demethylase 1 (LSD1, a repressive nuclear factor) reduces the response mediated by remaining NF-κB at the promoters responding to inflammatory stimuli. This refractory response in NPC cells might be a consequence of long-term exposure to microbes in vivo during carcinogenic progression. It may contribute to the decreased antitumor immune responses in NPC, among others despite heavy T-helper cell infiltration, and thus facilitate tumor progression.
|
|
| 10. |
- Chen, Cheng, et al.
(författare)
-
Performance of multiple satellite precipitation estimates over a typical arid mountainous area of China : Spatiotemporal patterns and extremes
- 2020
-
Ingår i: Journal of Hydrometeorology. - 1525-755X. ; 21:3, s. 533-550
-
Tidskriftsartikel (refereegranskat)abstract
- Precipitation in arid mountainous areas is characterized by low rainfall intensity and large spatial heterogeneity, which challenges satellite-based monitoring by the spaceborne sensors. This study aims to comparatively evaluate the detection ability of spatiotemporal patterns and extremes of rainfall by a range of mainstream satellite precipitation products [TMPA, Climate Hazards Group Infrared Precipitation with Station Data (CHIRPS), and PERSIANN-Climate Data Record (PERSIANN-CDR)] over a typical arid mountainous basin of China, benchmarking against rain gauge data from 2000 to 2015. Results showed that satellite precipitation estimates had relatively low accuracy at the daily scale, while a significant improvement of correlation coefficient (CC;>0.6) and a significant reduction of relative root-mean-square error (RRMSE;, 1.0) were found as time scale increases beyond the monthly scale. CHIRPS tended to overestimate the gauge precipitation with positive relative bias (RB), while the negative RBvalues for TMPAand PERSIANN-CDR indicated there was an underestimation. CHIRPS had the most similar spatial pattern and slope trends of the seasonal precipitation and interannual variations of annual precipitation with gauge observations. With the increase in rainfall rates, the probability of detection (POD) and critical success index (CSI) were reduced and the false alarm ratio (FAR) was increased significantly, demonstrating the limited capability for all the three satellite products for detecting heavy rainfall events. CHIRPS showed the best performance in detecting rainfall extremes compared to TMPA and PERSIANN-CDR, evidenced by the largerCSI values and similar extreme rainfall indices obtained from gauge records. This study provides valuable guidance for choosing satellite precipitation products instead of gauge observations for rainfall monitoring (especially rainfall extremes) and agricultural production management over arid mountainous area.
|
|
