| 1. |
- Zhang, Xiaoying, et al.
(författare)
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Expression of MMP-10 in lung cancer
- 2007
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Ingår i: Anticancer research. - 1791-7530. ; 27:4C, s. 2791-2795
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Tidskriftsartikel (refereegranskat)abstract
- Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and in the adjacent normal tissues. The ratio of mRNA levels in the tumor tissues compared to the adjacent normal tissues followed the pattern GAPDH > beta-actin > 18S rRNA. Thereafter, we chose 18S rRNA as the reference gene for MMP-10 mRNA level determinations. MMP-10 mRNA levels in tumor tissues were significantly lower than those in the adjacent normal tissues (p = 0.0423). However, the MMP- 10 protein levels were higher in the tumor tissues than in the adjacent normal tissues (p=0.0055). The MMP-10 mRNA level was positively-correlated to the MMP-10 protein level in tumor tissues (r=0.4672, p=0.0161), but this correlation was not seen in the adjacent normal tissues (r=-0.0030, p=0.9891). Conclusion: There were no statistical differences in MMP-10 mRNA levels and protein levels in relation to patient's gender, age, tumor stages, tumor size, lymph node metastasis or tumor histological type.
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| 2. |
- Guo, Xiao-Hua, et al.
(författare)
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Advanced glycation end products induce actin rearrangement and subsequent hyperpermeability of endothelial cells
- 2006
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Ingår i: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. - : Wiley. - 1600-0463 .- 0903-4641. ; 114:12, s. 874-883
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Tidskriftsartikel (refereegranskat)abstract
- This study aimed to determine the effects of advanced glycation end products (AGEs) on endothelial cytoskeleton morphology and permeability, and to detect the underlying signaling mechanisms involved in these responses. Cultured endothelial cells (ECs) were exposed to AGE-modified human serum albumin (AGE-HSA), and EC cytoskeletal changes were evaluated by observing fluorescence of F-actin following ligation with labeled antibodies. Endothelial permeability was detected by measuring the flux of TRITC-albumin across the EC monolayers. To explore the signaling pathways behind AGE-induced EC alteration, ECs were treated with either soluble anti-AGE receptor (RAGE) IgG, or the MAPK inhibitors PD98059 and SB203580 before AGE-HSA administration. To further elucidate possible involvement of the ERK and p38 pathways in AGE-induced EC changes, adenovirus-carried recombinant constitutive dominant-negative forms of upstream ERK and p38 kinases, namely MEK1(A) and MKK6b(A), were pre-infected into ECs 24 h prior to AGE-HSA exposure. AGE-HSA induced actin cytoskeleton rearrangement, as well as EC hyperpermeability, in a dose and time-dependent manner. The effects were attenuated in cells pretreated with anti-RAGE IgG, PD98059 or SB203580, respectively. EC pre-infection with MEK1(A) and MKK6b(A) also alleviated the effect of AGEs. Furthermore, adenovirus-mediated administration of activated forms of either MEK1 or MKK6b alone induced rearrangement of F-actin and hyperpermeability. The results indicate that ERK and p38 MAPK play important roles in the mediation of AGE-induced EC barrier dysfunction associated with morphological changes of the F-actin.
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| 3. |
- Jaskorzynska, Bozena, et al.
(författare)
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Dielectric and Plasmon Slot Waveguides for Photonic Integration
- 2009
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Ingår i: ICTON 2009: 11th International Conference on Transparent Optical Networks. - NEW YORK : IEEE. ; , s. 653-656
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Konferensbidrag (refereegranskat)abstract
- Slot waveguides formed either in high-index dielectrics or in metals attract great interest because they provide sub-wavelength confinement in the slot region. While this feature is very attractive for devices relying on stimulated emission or nonlinear effects, it does not necessarily improve the integration density. The spacing between dielectric slot waveguides is still limited by diffraction. Although for metal (plasmon) waveguides the total field can be shrunk far beyond the diffraction limit, the associated increase in propagation loss will set practical limits on both the minimum waveguide width and edge-to-edge separation. Here we compare the packing densities for 3D slot waveguides in silicon and plasmon waveguides in gold with a silicon slot. As a reference we also consider silicon photonic wire. We calculate center-to-center waveguide separations (pitch) versus cross-talk level. We show that at ca 24 dB/mu m cross-talk and requiring the attenuation length of at least 5 mu m, plasmon slot waveguides can be packed ca 3.5 times denser than silicon slot waveguides, and ca 2.5 times denser than photonic wires. We also show examples of the fabricated devices.
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| 4. |
- Jiang, Jingting, et al.
(författare)
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Clinical Evaluation of Serum Alpha-Fetoprotein-IgM Immune Complexes on the Diagnosis of Primary Hepatocellular Carcinoma
- 2009
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Ingår i: Journal of Clinical Laboratory Analysis. - : Wiley. - 1098-2825 .- 0887-8013. ; 23:4, s. 213-218
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Tidskriftsartikel (refereegranskat)abstract
- We evaluated clinical significance of serum alpha-fetoprotein (AFP)-IgM immune complexes, in comparison with free AFP, on the diagnosis of primary hepatocellular carcinoma (HCC). Serum levels of AFP-IgM immune complexes and free AFP were determined by the ELISA method and electrochemiluminescence, respectively, in 103 healthy controls, 74 patients suffering from primary HCC, 27 patients suffering from liver cirrhosis, and 63 patients suffering from chronic hepatitis. The best cut-off value of AFP-IgM and free AFP for diagnosis of primary HCC were 300 AU/mL and 10 mu g/L respectively, according to the area under the curve (AUC) in this study. The sensitivity of AFP-IgM and free AFP were 64.9 and 79.7%, and the specificity were 75.6 and 80.3%, respectively, when all cases of primary HCC were analyzed, and the AUC of free AFP was larger than that of AFP-IgM (0.85 vs. 0.72, Z = 3.21). However, in case of primary HCC at early stages (stages I and II) were analyzed, the AUC of AFP-IgM was larger than that of free AFP (0.91 vs. 0.82, Z = 1.73), which demonstrated that the sensitivity of AFP-IgM and free AFP were 94.4 and 72.2%, and the specificity were 81.9 and 79.9%, respectively. When both AFP-IgM and free AFP were positive, the specificity of diagnosis of primary HCC was 89.1%, and the efficacy was 79.0%. It is concluded that either sensitivity or specificity of serum level of AFP-IgM immune complexes was higher than that of free AFP in the diagnosis of primary HCC at early stages. As there was false positive AFP-IgM existed in the patients suffering from cirrhosis and chronic hepatitis, the combination of free AFP and AFP-IgM could significantly increase specificity and decrease false negative and/or false positive in the primary HCC at early stages. J. Clin. Lab. Anal. 23:213-218, 2009. (C) 2009 Wiley-Liss, Inc.
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| 5. |
- Song, Jun, et al.
(författare)
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Analysis method of the effect of fabrication errors on a planar waveguide demultiplexer
- 2006
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Ingår i: Optical and quantum electronics. - : Springer Science and Business Media LLC. - 0306-8919 .- 1572-817X. ; 38:15, s. 1203-1216
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Tidskriftsartikel (refereegranskat)abstract
- The impact of fabrication errors on a planar waveguide demultiplexer is analyzed based on an analytical method. The explicit expression of the transfer function taking into account phase and amplitude errors is presented in order to analyze the loss and crosstalk of the demultiplexer caused by fabrication errors. A basic requirement for the demultiplexer with a certain crosstalk criterion can be easily obtained. Using an etched diffraction grating demultiplexer as an example, it is shown that the analytical results have a good agreement with results from a numerical method.
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| 6. |
- Song, Jun, et al.
(författare)
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Design and fabrication of compact etched diffraction grating demultiplexers based on alpha-Si nanowire technology
- 2008
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Ingår i: Electronics Letters. - : Institution of Engineering and Technology (IET). - 0013-5194 .- 1350-911X. ; 44:13, s. 816-818
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Tidskriftsartikel (refereegranskat)abstract
- alpha-Silicon nanowire waveguides and related techniques are studied. An ultra-compact etched diffraction grating demultiplexer with 10 nm spacing is fabricated and characterised. The dimension of the device is around half a millimetre, which is one to two orders of magnitude smaller than conventional silica based devices.
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| 7. |
- Song, Jun, et al.
(författare)
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Design and fabrication of compact etched diffraction grating demultiplexers based on α-Si nanowire technology
- 2008
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Ingår i: 2008 IEEE/LEOS International Conference on Optical MEMS and Nanophotonics, OPT MEMS. - 9781424419180 ; , s. 148-149
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Konferensbidrag (refereegranskat)abstract
- Silicon nanowire waveguides and related etched diffraction grating (EDG) demultiplexers are studied by α-Si-on-SiO2 technology. Compact EDG demultiplexers with 10 run spacing for both echelle and total-internal- reflection (TIR) facets have been fabricated and characterized.
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| 8. |
- Song, Jun, et al.
(författare)
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Echelle grating demultiplexers with reduced return loss by using chirped diffraction order design
- 2006
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Ingår i: IEEE Photonics Technology Letters. - 1041-1135 .- 1941-0174. ; 18:14, s. 1506-1508
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Tidskriftsartikel (refereegranskat)abstract
- A design for echelle grating demultiplexers is presented to reduce the return loss. The input waveguide is placed on the minimal intensity position of the diffraction envelope. Then, by further chirping the diffraction order for each facet, we minimize the envelope intensity for other adjacent diffraction orders, which can contribute to a negligible return loss for a large spectral width. The present design is appropriate for multifrequency laser or multistage demultiplexer applications.
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| 9. |
- Song, Jun, et al.
(författare)
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Effects of surface roughness on the performance of an etched diffraction grating demultiplexer
- 2006
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Ingår i: Journal of the Optical Society of America A: Optics and Image Science, and Vision. - 1084-7529. ; 23:3, s. 646-650
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Tidskriftsartikel (refereegranskat)abstract
- The fabrication tolerance of surface roughness on the performance [such as the insertion loss, the polarization-dependent loss (PDL), and the chromatic dispersion] of an etched diffraction grating demultiplexer is analyzed by using an accurate method of moments. The results show that both the insertion loss and the chromatic dispersion rapidly increase as the roughness of the grating facets increases. Appropriate residual roughness of the shaded facets can reduce the PDL of the demultiplexer, and the mechanism for the existence of an optimal residual roughness is explained.
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| 10. |
- Song, Jun, et al.
(författare)
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Etched diffraction grating demultiplexers with large free-spectral range and large grating facets
- 2006
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Ingår i: IEEE Photonics Technology Letters. - 1041-1135 .- 1941-0174. ; 18:24, s. 2695-2697
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Tidskriftsartikel (refereegranskat)abstract
- A design for etched diffraction grating demultiplexers is presented to obtain both large grating facets and a larger free-spectral range (FSR) using the optimal chirped diffraction orders for different facets. The large grating facets by using a large diffraction order contribute to lowering the manufacturing difficulty, and can provide lower polarization-dependent loss. However, usually the large diffraction order must lower the FSR of the device by all means. Based on the present design, the FSR can overlay the whole diffraction envelop for a special order, but avoid the influence of the adjacent diffraction envelops. Calculations indicate that the extinction ratio between the operated order and adjacent orders can attain a 35 dB or so, which makes the crosstalk from channels in adjacent envelops to those in the operated diffraction envelop acceptable.
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