| 1. |
|
|
| 2. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 3. |
- Campbell, PJ, et al.
(författare)
-
Pan-cancer analysis of whole genomes
- 2020
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 578:7793, s. 82-
-
Tidskriftsartikel (refereegranskat)abstract
- Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale1–3. Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4–5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter4; identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation5,6; analyses timings and patterns of tumour evolution7; describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity8,9; and evaluates a range of more-specialized features of cancer genomes8,10–18.
|
|
| 4. |
- Xu, Jianqiang, et al.
(författare)
-
Design of Smart Unstaffed Retail Shop Based on IoT and Artificial Intelligence
- 2020
-
Ingår i: IEEE Access. - : Institute of Electrical and Electronics Engineers (IEEE). - 2169-3536. ; 8, s. 147728-147737
-
Tidskriftsartikel (refereegranskat)abstract
- Unstaffed retail shops have emerged recently and been noticeably changing our shopping styles. In terms of these shops, the design of vending machine is critical to user shopping experience. The conventional design typically uses weighing sensors incapable of sensing what the customer is taking. In the present study, a smart unstaffed retail shop scheme is proposed based on artificial intelligence and the internet of things, as an attempt to enhance the user shopping experience remarkably. To analyze multiple target features of commodities, the SSD (300x300) algorithm is employed; the recognition accuracy is further enhanced by adding sub-prediction structure. Using the data set of 18, 000 images in different practical scenarios containing 20 different type of stock keeping units, the comparison experimental results reveal that the proposed SSD (300x300) model outperforms than the original SSD (300x300) in goods detection, the mean average precision of the developed method reaches 96.1% on the test dataset, revealing that the system can make up for the deficiency of conventional unmanned container. The practical test shows that the system can meet the requirements of new retail, which greatly increases the customer flow and transaction volume.
|
|
| 5. |
- Feng, Yifan, et al.
(författare)
-
Fractal Superconducting Nanowire Single-Photon Detectors and Their Applications in Imaging
- 2022
-
Ingår i: Proceedings of the 2022 Conference on Lasers and Electro-Optics Pacific Rim, CLEO/PR 2022. - : Optica Publishing Group.
-
Konferensbidrag (refereegranskat)abstract
- We present our research on fractal superconducting nanowire single-photon detectors and their applications in light detection and ranging (LiDAR), full-Stokes polarimetric imaging, and non-line-of-sight imaging.
|
|
| 6. |
- Feng, Yifan, et al.
(författare)
-
Fractal Superconducting Nanowire Single-Photon Detectors and Their Applications in Imaging
- 2022
-
Ingår i: 2022 Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2022 - Proceedings. - : Institute of Electrical and Electronics Engineers Inc..
-
Konferensbidrag (refereegranskat)abstract
- We present our research on fractal superconducting nanowire single-photon detectors and their applications in light detection and ranging (LiDAR), full-Stokes polarimetric imaging, and non-line-of-sight imaging.
|
|
| 7. |
|
|
| 8. |
- Hu, Nan, et al.
(författare)
-
Full-Stokes polarimetric measurements and imaging using a fractal superconducting nanowire single-photon detector
- 2022
-
Ingår i: Optica. - : Optica Publishing Group. - 2334-2536. ; 9:4, s. 346-351
-
Tidskriftsartikel (refereegranskat)abstract
- Measuring the states of polarization (SoP) of light is fundamentally important for applications ranging from communication, sensing, spectroscopy, imaging, to navigation. Superconducting nanowire single-photon detectors (SNSPDs) are ideal detectors of choice for faint-light detection and measurements, but SNSPDs themselves cannot resolve the SoP of photons. Here, based on a fractal SNSPD, we demonstrate a full-Stokes polarimetric measurement system that can measure arbitrary SoP of faint light. The measured SoPs are in excellent agreement with those of a state-of-the-art commercial polarimeter, but the sensitivity of our system reaches -86.6 dBm, which is 26.6 dB better than that of the commercial counterpart. As a direct application, we further demonstrate remote polarimetric imaging (i.e., polarimetric LiDAR) with a complete set of polarimetric contrast.
|
|
| 9. |
- Hu, Nan, et al.
(författare)
-
Photon-Counting LIDAR Based on a Fractal SNSPD
- 2021
-
Ingår i: 2021 OPTICAL FIBER COMMUNICATIONS CONFERENCE AND EXPOSITION (OFC). - : IEEE.
-
Konferensbidrag (refereegranskat)abstract
- We report on a photon-counting LIDAR system based on a polarization-insensitive fractal SNSPD with high detection efficiency and high timing resolution, and showcase depth imaging of an object at 1560 nm with millimeter depth resolution.
|
|
| 10. |
- Hu, N., et al.
(författare)
-
Polarimetric Imaging Using One Fractal SNSPD
- 2022
-
Ingår i: 2022 Conference on Lasers and Electro-Optics, CLEO 2022. - : Institute of Electrical and Electronics Engineers Inc..
-
Konferensbidrag (refereegranskat)abstract
- We demonstrate a polarimetric imaging system with one fractal superconducting nanowire single-photon detector and time-multiplexed photon-counting scheme, which allows us to obtain images with multiple types of contrast.
|
|
