| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Sun, Huiliang, et al.
(författare)
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Reducing energy loss via tuning energy levels of polymer acceptors for efficient all-polymer solar cells
- 2020
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Ingår i: Science China Chemistry. - : Springer Science and Business Media LLC. - 1869-1870 .- 1674-7291. ; 63:12, s. 1785-1792
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Tidskriftsartikel (refereegranskat)abstract
- The open-circuit voltage (Voc) of all-polymer solar cells (all-PSCs) is typically lower than 0.9 V even for the most efficient ones. Large energy loss is the main reason for limiting Voc and efficiency of all-PSCs. Herein, through materials design using electron deficient building blocks based on bithiophene imides, the lowest unoccupied molecular orbital (LUMO) energy levels of polymer acceptors can be effectively tuned, which resulted in a reduced energy loss induced by charge generation and recombination loss due to the suppressed charge-transfer (CT) state absorption. Despite a negligible driving force, all-PSC based on the polymer donor and acceptor combination with well-aligned energy levels exhibited efficient charge transfer and achieved an external quantum efficiency over 10% while maintaining a large Voc of 1.02 V, leading to a 9.21% efficiency. Through various spectroscopy approaches, this work sheds light on the mechanism of energy loss in all-PSCs, which paves an avenue to achieving efficient all-PSCs with large Voc and drives the further development of all-PSCs.
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| 3. |
- Sun, Huiliang, et al.
(författare)
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Reducing energy lossviatuning energy levels of polymer acceptors for efficient all-polymer solar cells
- 2020
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Ingår i: Science China Chemistry. - : Science China Press and Springer-Verlag GmbH Germany. - 1674-7291 .- 1869-1870. ; 63, s. 1785-1792
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Tidskriftsartikel (refereegranskat)abstract
- The open-circuit voltage (V-oc) of all-polymer solar cells (all-PSCs) is typically lower than 0.9 V even for the most efficient ones. Large energy loss is the main reason for limitingV(oc)and efficiency of all-PSCs. Herein, through materials design using electron deficient building blocks based on bithiophene imides, the lowest unoccupied molecular orbital (LUMO) energy levels of polymer acceptors can be effectively tuned, which resulted in a reduced energy loss induced by charge generation and recombination loss due to the suppressed charge-transfer (CT) state absorption. Despite a negligible driving force, all-PSC based on the polymer donor and acceptor combination with well-aligned energy levels exhibited efficient charge transfer and achieved an external quantum efficiency over 10% while maintaining a largeV(oc)of 1.02 V, leading to a 9.21% efficiency. Through various spectroscopy approaches, this work sheds light on the mechanism of energy loss in all-PSCs, which paves an avenue to achieving efficient all-PSCs with largeV(oc)and drives the further development of all-PSCs.
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| 4. |
- Tang, Xiaopeng, et al.
(författare)
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Run-to-Run Control for Active Balancing of Lithium Iron Phosphate Battery Packs
- 2020
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Ingår i: IEEE Transactions on Power Electronics. - 0885-8993 .- 1941-0107. ; 35:2, s. 1499-1512
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Tidskriftsartikel (refereegranskat)abstract
- © 1986-2012 IEEE. Lithium iron phosphate battery packs are widely employed for energy storage in electrified vehicles and power grids. However, their flat voltage curves rendering the weakly observable state of charge are a critical stumbling block for charge equalization management. This paper focuses on the real-time active balancing of series-connected lithium iron phosphate batteries. In the absence of accurate in situ state information in the voltage plateau, a balancing current ratio (BCR) based algorithm is proposed for battery balancing. Then, BCR-based and voltage-based algorithms are fused, responsible for the balancing task within and beyond the voltage plateau, respectively. The balancing process is formulated as a batch-based run-to-run control problem, as the first time in the research area of battery management. The control algorithm acts in two timescales, including timewise control within each batch run and batchwise control at the end of each batch. Hardware-in-the-loop experiments demonstrate that the proposed balancing algorithm is able to release 97.1% of the theoretical capacity and can improve the capacity utilization by 5.7% from its benchmarking algorithm. Furthermore, the proposed algorithm can be coded in C language with the binary code in 118 328 bytes only and, thus, is readily implementable in real time.
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