| 1. |
- Børresen, A L, et al.
(author)
-
Prenatal diagnosis of glycerol-kinase deficiency associated with a DNA deletion on the short arm of the X-chromosome.
- 1987
-
In: Clinical genetics. - 0009-9163. ; 32:4, s. 254-9
-
Journal article (peer-reviewed)abstract
- Amniocentesis was performed in a woman who previously had given birth to a boy who died at 12 months of age with a diagnosis of glyceroluria and adrenal insufficiency. A high amount of glycerol (9.0 standard deviations above mean for controls) was found in the amniotic fluid. Enzyme activity of glycerol-kinase (ATP:glycerol-3-phosphotransferase, EC 2.7.1.30) in the cultured amniotic fluid cells was very low. The pregnancy was terminated and a male fetus was aborted. Examinations of DNA isolated from the fetus did demonstrate deletions of two out of 16 DNA probes mapping to the short arm of the X-chromosome. The probes failing to hybridize to DNA from the fetus were C7 (DXS28) and L1.4 (DXS68), both mapping to Xp21.3 and located terminal to the Duchenne locus.
|
|
| 2. |
- Sjöberg, Lennart, et al.
(author)
-
A comparison between Bactec aerobic resin and hypertonic blood culture media.
- 1988
-
In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). - : Wiley. - 0903-4641 .- 1600-0463. ; 96:8, s. 720-722
-
Journal article (peer-reviewed)abstract
- The presence of antimicrobial agents in patients' blood is thought to represent an important source of false-negative blood cultures. This has led to the incorporation of agents with inhibitory effects on antimicrobial drugs into culture medium. In the present study, Bactec aerobic resin-containing blood culture medium was compared with Bactec hypertonic blood culture medium. 504 patients receiving cytostatic and/or antibiotic treatment were studied. Sensitivity calculations on detection of bacteremia in these patients gave 0.91 for the resin medium and 0.79 for the hypertonic blood culture system and showed a significant difference (p = 0.016). In addition, the resin-containing system more rapidly detected positive cultures than the hypertonic system.
|
|
| 3. |
- Wirth, Michael, 1956, et al.
(author)
-
Interactions between DNA and mono-, bis-, tris-, tetrakis-, and hexakis(aminoacridines). A linear and circular dichroism, electric orientation relaxation, viscometry, and equilibrium study
- 1988
-
In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 110:3, s. 932-939
-
Journal article (peer-reviewed)abstract
- The interaction between DNA and a series of mono-, bis-, tris-, tetrakis-, and hexakis-intercalating 9-aminoacridines has been studied with flow linear dichroism (LD), circular dichroism (CD), electric orientation relaxation (EOR) techniques, and with viscometry and equilibrium analyses. The orientation of the 9-aminoacridine ligand relative to the average orientation of the DNA bases, measured by LD, shows that with both 9-aminoacridine and the bis(acridines) the in-plane short axes of the acridine ligands are oriented perfectly parallel to the planes of the DNA bases, as expected for classical intercalation, whereas the long axes are found to be significantly tilted. This is supported by the DNA lengthening measured by EOR, which for 9-aminoacridine is 1.5 base-pair units, compared with 1.0 for ethidium bromide. Also in case of the tris(acridines) LD, CD, viscometry, and equilibrium data indicate that all acridine ligands are intercalated. The binding analysis shows an increasing degree of cooperativity in the sequence 9-aminoacridine < bis(acridines) < tris(acridines), and the corresponding binding densities, 4, 8, and 11–14, respectively, are in good agreement with those expected from the nearest-neighbor exclusion principle. The LD and CD measurements show that the tetrakis- and hexakis(acridines), despite long and flexible links, bind to DNA with only three of the acridine ligands intercalated.
|
|
| 4. |
- Nilsonne, Åsa, et al.
(author)
-
Measuring the rate of change of voice fundamental frequency in fluent speech during mental depression
- 1988
-
In: The Journal of the Acoustical Society of America. - : American Institute of Physics (AIP). - 0001-4966. ; 83:2, s. 716-728
-
Journal article (peer-reviewed)abstract
- A method of measuring the rate of change of fundamental frequency has been developed in an effort to find acoustic voice parameters that could be useful in psychiatric research. A minicomputer program was used to extract seven parameters from the fundamental frequency contour of tape‐recorded speech samples: (1) the average rate of change of the fundamental frequency and (2) its standard deviation, (3) the absolute rate of fundamental frequency change, (4) the total reading time, (5) the percent pause time of the total reading time, (6) the mean, and (7) the standard deviation of the fundamental frequency distribution. The method is demonstrated on (a) a material consisting of synthetic speech and (b) voice recordings of depressed patients who were examined during depression and after improvement.
|
|
| 5. |
|
|
| 6. |
- Gustafsson, Hans, 1952-
(author)
-
Salivary gland neoplasms : studies on the cytoskeleton, the secretory apparatus and the nuclear DNA content
- 1986
-
Doctoral thesis (other academic/artistic)abstract
- The heterogeneity of salivary gland neoplasms have made classification and prognostication of these tumours sometimes difficult, and the introduction of techniques, such as enzyme and carbohydrate histochemistry and electron microscopy have only to a certain extent increased our knowledge in these respects. In the present study immunohistochemical methods have been used to identify intermediate filament proteins (IFP) in normal fetal and adult parotid glands, as well as in salivary neoplasms. The intermediate filaments (IF) make up the cytoskeleton in eucaryotic cells. Epithelial tissue contains IF composed of different cytokeratins (CK 1-19) whilst mesenchymal tissue generally contains IF composed of vimentin, and the IFP pattern is very stable even during cell transformation. It would thus be possible to further clarify the histogenesis of salivary neoplasms by identifying IFP, in addition the IFP pattern would probably be useful in tumour typing. Furthermore, ultrastructural cytochemical studies, microspectorphotometry on nuclear DNA as well as enzyme secretory studies of certain tumour types were carried out, in order to further characterize the biology of salivary neoplasms.The immunohistochemical investigations showed that in normal parotid tissue, the different cell types differed in IFP expression: acinar cells express mainly CK 18 and myoepithelial cells mainly CK 17 and 19, whilst duct cells contained a broad range of CK. Vimentin could in addition to CK be detected in myoepithelial cells and basal cells of excretory ducts. Fetal parotid cells showed a similar CK pattern as mature duct cells. In addition, vimentin could be found in some basal cells of the terminal tubules of the fetal glands. Salivary neoplasms could be divided into three types with regard to their IFP pattern: Acinic cell carcinomas showed a CK-pattern similar to normal acinar cells but a co-expression of CK and vimentin was present in some cells. Adenoid cystic carcinomas, mixed tumours and basal cell adenomas showed a CK-pattern of normal duct or myoepithelial cells. The peripheral cells were also vimentin positive. 3. Mucoepidermoid carcinomas and adenocarcinomas had a similar CK-pattern as duct cells, and no tumour cells contained vimentin. This indicates that typing of IFP may be useful for subgrouping of salivary neoplasms.By stereological measurements, the cells of acinic cell carcinomas were found to be very similar to normal parotid acinar cells. Furthermore, they contained amylase and after stimulation by norepiphrine a secretory response was induced, with a rise in intracellular cAMP as well as a release of amylase. By single cell measurements of nuclear DNA content, no difference was found between acinic cell carcinomas with definite metastasis and those without recurrence, both in paraffin sections and cytological smears.
|
|
| 7. |
|
|
| 8. |
- Stenling, Roger
(author)
-
The enterocyte in small intestinal adaption : an experimental and clinicopathological study with special reference to the ultrastructure of the brush border
- 1984
-
Doctoral thesis (other academic/artistic)abstract
- The small intestine mucosa is known to be able to adapt itself to several kinds of both physiological and pathological conditions. The adaptive patterns of the structure of the enterocytes, particularly their apical surface (brush border), were studied in three models: (1) in rats, subjected to antrectomy or antral exclusion, combined with gastroduodenostomy and gastrojejunostomy; (2) in rats with alloxan diabetes; (3) in children with coeliac disease; a) in its active phase; b) after long-term treatment with gluten-free diets; c) after long-term challenge with dietary gluten following treatment; d) after short-term elimination of dietary gluten. Gut mucosa from fasting or fed, normal or sham-operated rats, fasting cats, and short-statured children with no signs of gastrointestinal disease served as controls. - The specimens were prepared for light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Quantitation of structural variables was achieved by means of LM and TEM morphometrical procedures.Differentiation of the rat enterocytes from the base to the crest of the villi was structurally reflected by doubling of their apical cell area, an increase in cell height, and a decrease of both nuclear and mitochondrial volume densities. In mature normal rat enterocytes, high-power SEM showed regularly arranged, nude microvilli in thir apical surfaces, whereas in cat and man the apical surfaces were covered by a thick glycocalyx. - Fasting for 24 hours decreased the total length of the rat small intestine and the height of the enterocytes. Antrectomy and antral exclusion with gastrojejunostomy produced an increase of the apical surfaces of the enterocytes of the seif-emptying duodenal blind loop, whereas no changes occurred after antrectomy with gastroduodenostomy. In the jejunum, the apical surface area was reduced both after antrectomy and antral exclusion. In the diabetic rats a slight decrease of the apical surface area, together with an elongation of both the villi and the crypts, was observed in the jejunum, whereas no structural changes occurred in the duodenal mucosa.Both in active coeliac disease and after long-term challenge with dietary gluten, SEM analyses showed uniformly destructed villi. The apical surfaces of the enterocytes were frequently convex and irregular in size and delineation (the surface of the normal enterocytes was polygonal and flat). Ultrastructurally, the apical surfaces were severely damaged with a distortion of the glycocalyx and with marked irregularity of the microvilli. - After gluten elimination, the surface ultrastructure of the enterocytes in the coeliac gut mucosa generally showed a rapid, clear-cut restoration despite a remaining severe atrophy of the villi. Successful dietary treatment (after about one year of gluten-free diet) restored the small intestine mucosa to normal as assessed both by LM and low-power SEM. In contrast, high-power SEM often disclosed persisting lesions of the enterocytes. Another provocation with gluten for up to 9 days in clinically healed coeliac mucosa did not significantly alter the surface ultrastructure of the enterocytes.
|
|
| 9. |
|
|
| 10. |
- Ask, Per, et al.
(author)
-
Combined pH and pressure measurement device for oesophageal investigations.
- 1981
-
In: Medical and Biological Engineering and Computing. - 0140-0118 .- 1741-0444. ; 19:4, s. 443-446
-
Journal article (peer-reviewed)abstract
- A combined pH- and pressure-measurement device for oesophageal investigations has been designed using monocrystalline antimony pH electrodes and perfused polyvinyl catheters. The combined device facilitates pressure measurements simultaneously with pH recording, both distal and proximal to the pH electrode. The device is easier to pass through the nose to the oesophagus than the conventional glass pH electrode. pH and pressure measurements in the oesophagus are therefore simplified and valuable information about the function of the region of the lower oesophageal sphincter is added owing to the simultaneous recording of the two parameters.
|
|
