Sökning: id:"swepub:oai:DiVA.org:kth-10913" >
Proliferation and v...
Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip
-
- Hultström, Jessica (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Biomedical & X-Ray Physics
-
- Manneberg, Otto (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
-
- Dopf, Katja (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Biomedical & X-Ray Physics
-
visa fler...
-
- Hertz, Hans M. (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Biomedical & X-Ray Physics
-
- Brismar, Hjalmar (författare)
- Karolinska Institutet,KTH,Cellens fysik,Cell Physics
-
- Wiklund, Martin (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Biomedical & X-Ray Physics
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
-
Ingår i: Ultrasound in Medicine and Biology. - : Elsevier BV. - 0301-5629 .- 1879-291X. ; 33, s. 145-151
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Ultrasonic-standing-wave (USW) technology has potential to become a standard method for gentle and contactless cell handling in microfluidic chips. We investigate the viability of adherent cells exposed to USWs by studying the proliferation rate of recultured cells following ultrasonic trapping and aggregation of low cell numbers in a microfluidic chip. The cells form 2-D aggregates inside the chip and the aggregates are held against a continuous flow of cell culture medium perpendicular to the propagation direction of the standing wave. No deviations in the doubling time from expected values (24 to 48 h) were observed for COS-7 cells held in the trap at acoustic pressure amplitudes up to 0.85 MPa and for times ranging between 30 and 75 min. Thus, the results demonstrate the potential of ultrasonic standing waves as a tool for gentle manipulation of low cell numbers in microfluidic systems.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- mammalian-cells; retention; sedimentation; aggregation; perfusion; channels; driven; matrix; filter; trap
- Bioengineering
- Bioteknik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas