Sökning: id:"swepub:oai:DiVA.org:kth-19474" >
An active-site titr...
An active-site titration method for lipases
-
Rotticci, D. (författare)
-
Norin, T. (författare)
-
- Hult, Karl (författare)
- KTH,Biokemi och biokemisk teknologi
-
visa fler...
-
- Martinelle, Mats (författare)
- KTH,Biokemi och biokemisk teknologi
-
visa färre...
-
(creator_code:org_t)
- 2000
- 2000
- Engelska.
-
Ingår i: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. - 1388-1981 .- 1879-2618. ; 1483:1, s. 132-140
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A method for active-site titration of lipases has been developed based on irreversible inhibition by methyl p-nitrophenyl n-hexylphosphonate. This method was applied to five lipases displaying from minor to pronounced interfacial activation. Soluble and immobilized lipases were successfully titrated in aqueous media. A low concentration of sodium dodecyl sulfate was needed for lipases displaying pronounced interfacial activation. The carrier of some of the immobilized preparations adsorbed part of the produced p-nitrophenolate, This problem could be solved by extracting the p-nitrophenolate after inhibition. The method was extended to apolar organic solvents in the case of immobilized lipase preparations.
Nyckelord
- concentration
- immobilized lipase
- aqueous medium
- organic medium
- interfacial activation
- detergent
- inhibitor
- serine proteases
- interfacial activation
- organic-chemistry
- in-vitro
- biocatalysts
- inhibition
- solvents
- cutinase
- titrant
- enzymes
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas