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High-content nucleu...
High-content nucleus based 3D image cytometery of whole multicellular tumour spheroids
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- Olofsson, Karl (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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- Carannante, Valentina (författare)
- Karolinska Institutet, Sweden
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- Önfelt, Björn (författare)
- KTH,Biomedicinsk fysik och röntgenfysik,Karolinska Institutet, Sweden
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visa fler...
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- Wiklund, Martin (författare)
- KTH,Biomedicinsk fysik och röntgenfysik
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visa färre...
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(creator_code:org_t)
- Chemical and Biological Microsystems Society, 2018
- 2018
- Engelska.
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Ingår i: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - : Chemical and Biological Microsystems Society. ; , s. 1433-1434
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We have previously presented an ultrasonic standing wave (USW) based multicellular tumor spheroid (MCTS) culture platform where 100 MCST can be formed, processed and imaged while being retained in micro-wells [1]. This platform was used to show MCTS characterization based on on-chip microscopy and off-chip flow cytometry [2]. Here, we present a high-content 3D image cytometry method of whole MCTS based on DNA staining where spatial and functional information can be assessed.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Confocal imaging
- High-content cytometry
- Image analysis
- Multicellular tumor spheroids
- 3-D image
- Cytometry
- Functional information
- High-content
- Micro wells
- Ultrasonic standing waves
- Tumors
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)