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Targeted proteomics...
Targeted proteomics analysis of plasma proteins using recombinant protein standards for addition only workflows
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- Kotol, David (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Hober, Andreas, 1992- (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Strandberg, Linnéa (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Svensson, Anne-Sophie (författare)
- KTH,Proteinvetenskap
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- Uhlén, Mathias (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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- Edfors, Fredrik (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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(creator_code:org_t)
- Future Science Ltd, 2021
- 2021
- Engelska.
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Ingår i: BioTechniques. - : Future Science Ltd. - 0736-6205 .- 1940-9818. ; 71:3, s. 473-483
- Relaterad länk:
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https://doi.org/10.2...
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https://doi.org/10.2...
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Ämnesord
Stäng
- Targeted proteomics is an attractive approach for the analysis of blood proteins. Here, we describe a novel analytical platform based on isotope-labeled recombinant protein standards stored in a chaotropic agent and subsequently dried down to allow storage at ambient temperature. This enables a straightforward protocol suitable for robotic workstations. Plasma samples to be analyzed are simply added to the dried pellet followed by enzymatic treatment and mass spectrometry analysis. Here, we show that this approach can be used to precisely (coefficient of variation <10%) determine the absolute concentrations in human plasma of hundred clinically relevant protein targets, spanning four orders of magnitude, using simultaneous analysis of 292 peptides. The use of this next-generation analytical platform for high-throughput clinical proteome profiling is discussed.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- blood plasma
- internal standards
- mass spectrometry
- multiplex analysis
- plasma profiling
- room temperature storage
- sample preparation
- stability
- stable Isotope standards
- targeted proteomics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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