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The role of the 5' ...
The role of the 5' sensing function of ribonuclease E in cyanobacteria
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- Hoffmann, Ute A. (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab,Univ Freiburg, Inst Biol 3, Mol Genet Prokaryotes, Freiburg, Germany.
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- Lichtenberg, Elisabeth (författare)
- Univ Freiburg, Inst Biol 3, Mol Genet Prokaryotes, Freiburg, Germany.
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- Rogh, Said N. (författare)
- Univ Freiburg, Inst Biol 3, Mol Genet Prokaryotes, Freiburg, Germany.
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- Bilger, Raphael (författare)
- Univ Freiburg, Fac Biol, Genet & Expt Bioinformat, Freiburg, Germany.
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- Reimann, Viktoria (författare)
- Univ Freiburg, Fac Biol, Genet & Expt Bioinformat, Freiburg, Germany.
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- Heyl, Florian (författare)
- Univ Freiburg, Dept Comp Sci, Bioinformat Grp, Freiburg, Germany.
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- Backofen, Rolf (författare)
- Univ Freiburg, Dept Comp Sci, Bioinformat Grp, Freiburg, Germany.;Univ Freiburg, Signalling Res Ctr BIOSS & CIBSS, Freiburg, Germany.
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- Steglich, Claudia (författare)
- Univ Freiburg, Fac Biol, Genet & Expt Bioinformat, Freiburg, Germany.
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- Hess, Wolfgang R. (författare)
- Univ Freiburg, Fac Biol, Genet & Expt Bioinformat, Freiburg, Germany.
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- Wilde, Annegret (författare)
- Univ Freiburg, Inst Biol 3, Mol Genet Prokaryotes, Freiburg, Germany.;Univ Freiburg, Inst Biol 3, Mol Genet Prokaryotes, D-79104 Freiburg, Germany.
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(creator_code:org_t)
- Informa UK Limited, 2024
- 2024
- Engelska.
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Ingår i: RNA Biology. - : Informa UK Limited. - 1547-6286 .- 1555-8584. ; 21:1, s. 1-18
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- RNA degradation is critical for synchronising gene expression with changing conditions in prokaryotic and eukaryotic organisms. In bacteria, the preference of the central ribonucleases RNase E, RNase J and RNase Y for 5'-monophosphorylated RNAs is considered important for RNA degradation. For RNase E, the underlying mechanism is termed 5' sensing, contrasting to the alternative 'direct entry' mode, which is independent of monophosphorylated 5' ends. Cyanobacteria, such as Synechocystis sp. PCC 6803 (Synechocystis), encode RNase E and RNase J homologues. Here, we constructed a Synechocystis strain lacking the 5' sensing function of RNase E and mapped on a transcriptome-wide level 283 5'-sensing-dependent cleavage sites. These included so far unknown targets such as mRNAs encoding proteins related to energy metabolism and carbon fixation. The 5' sensing function of cyanobacterial RNase E is important for the maturation of rRNA and several tRNAs, including tRNAGluUUC. This tRNA activates glutamate for tetrapyrrole biosynthesis in plant chloroplasts and in most prokaryotes. Furthermore, we found that increased RNase activities lead to a higher copy number of the major Synechocystis plasmids pSYSA and pSYSM. These results provide a first step towards understanding the importance of the different target mechanisms of RNase E outside Escherichia coli.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- RNA degradation
- RNase E
- cyanobacteria
- Synechocystis
- 5' sensing
- RNA-seq
- tRNA maturation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Hoffmann, Ute A.
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Lichtenberg, Eli ...
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Rogh, Said N.
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Bilger, Raphael
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Reimann, Viktori ...
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Heyl, Florian
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visa fler...
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Backofen, Rolf
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Steglich, Claudi ...
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Hess, Wolfgang R ...
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Wilde, Annegret
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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RNA Biology
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Kungliga Tekniska Högskolan