Derivation of a reversible Hill equation with modifiers affecting catalytic properties

• An existing generic enzyme rate equation, the reversible Hill equation, was generalized to account for modifiers affecting the catalytical properties of the enzyme as well as for the case of several substrates and products. The resulting generalized reversible Hill (GRH) equation has relatively few but operationally well-defined parameters. Its usefulness is demonstrated by fitting it to experimental data on mammalian muscle phosphofructokinase. The fit is superior to that of previous models to the same data. The rate equation derived is suitable for replacing more complicated rate equations when exact mechanisms are unknown and data is scarce or contradictory.

Subject headings

NATURVETENSKAP  -- Data- och informationsvetenskap (hsv//swe)

NATURAL SCIENCES  -- Computer and Information Sciences (hsv//eng)

Keyword

Metabolic Modeling

Enzyme Kinetics

Reversible Hill Equation

Operationally Welldefined parameters

Phosphofructokinase

Publication and Content Type

ref (subject category)

art (subject category)