Sökning: id:"swepub:oai:DiVA.org:liu-100011" >
Non-genotoxic carci...
Non-genotoxic carcinogen exposure induces defined changes in the 5-hydroxymethylome
-
- Thomson, John P (författare)
- University of Edinburgh, Western General Hospital, UK
-
- Lempiäinen, Harri (författare)
- Novartis Institute for Biomedical Research, Basel, Switzerland
-
- Hackett, Jamie A (författare)
- University of Edinburgh, Western General Hospital, UK
-
visa fler...
-
- Nestor, Colm E, 1977- (författare)
- University of Edinburgh, Western General Hospital, Edinburgh, UK
-
- Müller, Arne (författare)
- Novartis Institute for Biomedical Research, Basel, Switzerland
-
- Bolognani, Federico (författare)
- Novartis Institute for Biomedical Research, Basel, Switzerland
-
- Oakeley, Edward J (författare)
- Novartis Institutes for Biomedical Research, Basel, Switzerland
-
- Schübeler, Dirk (författare)
- Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
-
- Terranova, Rémi (författare)
- Novartis Institute for Biomedical Research, Basel, Switzerland
-
- Reinhardt, Diana (författare)
- University of Edinburgh, Western General Hospital, UK
-
- Moggs, Jonathan G (författare)
- Novartis Institute for Biomedical Research, Basel, Switzerland
-
- Meehan, Richard R (författare)
- University of Edinburgh, Western General Hospital, UK
-
visa färre...
-
(creator_code:org_t)
- BioMed Central, 2012
- 2012
- Engelska.
-
Ingår i: Genome Biology. - : BioMed Central. - 1465-6906 .- 1474-760X. ; 13:10
- Relaterad länk:
-
https://liu.diva-por... (primary) (Raw object)
-
visa fler...
-
https://genomebiolog...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: Induction and promotion of liver cancer by exposure to non-genotoxic carcinogens coincides with epigenetic perturbations, including specific changes in DNA methylation. Here we investigate the genome-wide dynamics of 5-hydroxymethylcytosine (5hmC) as a likely intermediate of 5-methylcytosine (5mC) demethylation in a DNA methylation reprogramming pathway. We use a rodent model of non-genotoxic carcinogen exposure using the drug phenobarbital.RESULTS:Exposure to phenobarbital results in dynamic and reciprocal changes to the 5mC/5hmC patterns over the promoter regions of a cohort of genes that are transcriptionally upregulated. This reprogramming of 5mC/5hmC coincides with characteristic changes in the histone marks H3K4me2, H3K27me3 and H3K36me3. Quantitative analysis of phenobarbital-induced genes that are involved in xenobiotic metabolism reveals that both DNA modifications are lost at the transcription start site, while there is a reciprocal relationship between increasing levels of 5hmC and loss of 5mC at regions immediately adjacent to core promoters.CONCLUSIONS:Collectively, these experiments support the hypothesis that 5hmC is a potential intermediate in a demethylation pathway and reveal precise perturbations of the mouse liver DNA methylome and hydroxymethylome upon exposure to a rodent hepatocarcinogen.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Thomson, John P
-
Lempiäinen, Harr ...
-
Hackett, Jamie A
-
Nestor, Colm E, ...
-
Müller, Arne
-
Bolognani, Feder ...
-
visa fler...
-
Oakeley, Edward ...
-
Schübeler, Dirk
-
Terranova, Rémi
-
Reinhardt, Diana
-
Moggs, Jonathan ...
-
Meehan, Richard ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Genetik
- Artiklar i publikationen
-
Genome Biology
- Av lärosätet
-
Linköpings universitet