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Assessment of gluco...
Assessment of glucose oxidase behaviour in alcoholic solutions using disposable electrodes
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- Kroger, S (författare)
- Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Setford, SJ (författare)
- Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Turner, APF (författare)
- Cranfield University, UK
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(creator_code:org_t)
- Elsevier Science B.V., Amsterdam. 1998
- 1998
- Engelska.
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Ingår i: Analytica Chimica Acta. - : Elsevier Science B.V., Amsterdam.. - 0003-2670 .- 1873-4324. ; 368:3, s. 219-231
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Solvent resistant screen-printed three-electrode devices were employed to assess the behaviour of free and immobilised glucose oxidase in water-miscible organic solvent/aqueous buffer mixtures. Three alcoholic solvents were examined, methanol, ethanol and isopropanol. A rhodinised-carbon electrocatalyst was employed to facilitate hydrogen peroxide oxidation at a decreased operating potential. The devices had a linear response range of 1-10 mU GOX and allowed detection of down to 2 x 10(-14) mol GOX. The measurement of 0-1 (v/v) glucose in 30% (v/v) ethanol was achieved in 2 min using these highly storage-stable enzyme electrodes. Such devices are likely to have widespread applications in the diagnostic and on-line monitoring fields where rapid analysis in alcoholic matrices is desirable. (C) 1998 Elsevier Science B.V. All rights reserved.
Nyckelord
- glucose oxidase; screen-printed electrodes; alcoholic solutions; organic phase; amperometric biosensors; glucose measurement
- TECHNOLOGY
- TEKNIKVETENSKAP
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