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Mouse corneal lymph...
Mouse corneal lymphangiogenesis model.
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- Cao, Renhai (författare)
- Karolinska Institutet
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- Lim, Sharon (författare)
- Karolinska Institutet
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- Ji, Hong (författare)
- Karolinska Institutet
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- Zhang, Yin (författare)
- Karolinska Institutet
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- Yang, Yunlong (författare)
- Karolinska Institutet
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- Honek, Jennifer (författare)
- Karolinska Institutet
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- Hedlund, Eva-Maria (författare)
- Karolinska Institutet
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- Cao, Yihai (författare)
- Linköpings universitet,Fysiologi,Hälsouniversitetet
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(creator_code:org_t)
- 2011-05-19
- 2011
- Engelska.
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Ingår i: Nature protocols. - : Springer Science and Business Media LLC. - 1750-2799 .- 1754-2189. ; 6:6, s. 817-26
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- This protocol describes a powerful in vivo method to quantitatively study the formation of new lymphatic vessels in the avascular cornea without interference of pre-existing lymphatics. Implantation of 100 ng of lymphangiogenic factors such as vascular endothelial growth factor (VEGF)-A, VEGF-C or fibroblast growth factor-2, together with slow-release polymers, into a surgically created micropocket in the mouse cornea elicits a robust lymphangiogenic response. Newly formed lymphatic vessels are detected by immunohistochemical staining of the flattened corneal tissue with lymphatic endothelial-specific markers such as lymphatic vessel endothelial hyaluronan receptor-1; less-specific markers such as vascular endothelial growth factor receptor 3 may also be used. Lymphatic vessel growth in relation to hemangiogenesis can be readily detected starting at day 5 or 6 after pellet implantation and persists for ∼14 d. This protocol offers a unique opportunity to study the mechanisms underlying lymphatic vessel formation, remodeling and function.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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