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Polysaccharides fro...
Polysaccharides from chayote enhance lipid efflux and regulate NLRP3 inflammasome priming in macrophage-like THP-1 cells exposed to cholesterol crystals
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- Castro Alves, Victor, 1986- (författare)
- Department of Food Science and Experimental Nutrition, University of São Paulo, São Paulo, Brazil; Food Research Center (FoRC), CEPID-FAPESP, São Paulo, Brazil
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- Misuzu Shiga, Tânia (författare)
- Department of Food Science and Experimental Nutrition, University of São Paulo, São Paulo, Brazil; Food Research Center (FoRC), CEPID-FAPESP, São Paulo, Brazil
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- Oliveira do Nascimento, João R. (författare)
- Department of Food Science and Experimental Nutrition, University of São Paulo, São Paulo, Brazil; Food Research Center (FoRC), CEPID-FAPESP, São Paulo, Brazil; Food and Nutrition Research Center (NAPAN), University of São Paulo, São Paulo, Brazil
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(creator_code:org_t)
- Elsevier, 2019
- 2019
- Engelska.
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 127, s. 502-510
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The contribution of dietary fiber to decrease the risk of atherosclerosis may occur through other mechanisms besides the increased excretion of cholesterol. Although macrophages are crucial for lipid clearance, the excessive uptake of cholesterol crystals (CC) by these cells induce NLRP3 inflammasome and foam cell formation. Thus, we investigated whether the water-soluble DF from chayote (WSP) regulate CC-pretreated macrophage-like THP-1 cells. Linkage analysis indicated that WSP is composed mainly of pectic homogalacturonan and highly branched type I rhamnogalacturonan as well as hemicellulosic material including glucomannan, xyloglucan, and glucurono(arabino)xylan. WSP reduced interleukin (IL)-1β and chemokine release in CC-pretreated macrophages. Notably, WSP also reduced lipid accumulation in cells previously exposed to CC. Furthermore, WSP upregulated liver X receptor alpha expression, which may account for increased lipid efflux, and reduced matrix metallopeptidase 9 expression. WSP also reduced active caspase-1 protein levels, and downregulated NLRP3 and IL-1β gene expression in CC-pretreated cells, suggesting that this polysaccharide fraction regulates the priming signals required for NLRP3 inflammasome activation. Thus, WSP regulate lipid efflux and suppress inflammasome priming in macrophages, suggesting that the health benefits of this dietary fiber could go beyond its physical properties on the gastrointestinal tract.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Atherosclerosis
- Cholesterol crystals
- Dietary fiber
- Inflammasome
- Liver X receptor
- Sechium edule
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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