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Identification and ...
Identification and characterization of a Drosophila nuclear proteasome activator : a homolog of human 11S REggamma (PA28gamma)
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- Masson, Patrick (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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Andersson, Oskar (author)
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Petersen, Ulla-Maya (author)
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- Young, Patrick (author)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 276:2, s. 1383-1390
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- We report the cloning and characterization of aDrosophila proteasome 11 S REGγ (PA28) homolog. The 28-kDa protein shows 47% identity to the human REGγ and strongly enhances the trypsin-like activities of both Drosophila and mammalian 20 S proteasomes. Surprisingly, the DrosophilaREG was found to inhibit the proteasome's chymotrypsin-like activity against the fluorogenic peptide succinyl-LLVY-7-amino-4-methylcoumarin. Immunocytological analysis reveals that the Drosophila REG is localized to the nucleus but is distributed throughout the cell when nuclear envelope breakdown occurs during mitosis. Through site-directed mutagenesis studies, we have identified a functional nuclear localization signal present in the homolog-specific insert region. TheDrosophila PA28 NLS is similar to the oncogene c-Myc nuclear localization motif. Comparison between uninduced and innate immune induced Drosophila cells suggests that the REGγ proteasome activator has a role independent of the invertebrate immune system. Our results support the idea that γ class proteasome activators have an ancient conserved function within metazoans and were present prior to the emergence of the α and β REG classes.
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- art (subject category)
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