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ELOVL2 controls the...
ELOVL2 controls the level of n-6 28:5 and 30:5 fatty acids in testis : a prerequisite for male fertility and sperm maturation in mice
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- Zadravec, Damir (författare)
- Stockholms universitet,Avdelningen för fysiologi
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Tvrdik, Petr (författare)
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Guillou, Hervé (författare)
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Haslam, Richard (författare)
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- Kobayashi, Tsutomu (författare)
- Stockholms universitet,Wenner-Grens institut
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Napier, Johnathan A (författare)
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Capecchi, Mario R (författare)
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- Jacobsson, Anders (författare)
- Stockholms universitet,Avdelningen för fysiologi
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(creator_code:org_t)
- 2011
- 2011
- Engelska.
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Ingår i: Journal of Lipid Research. - 0022-2275 .- 1539-7262. ; 52:2, s. 245-255
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- ELOVL2 is a member of the mammalian microsomal ELOVL fatty acid enzyme family, involved in the elongation of very long-chain fatty acids including PUFAs required for various cellular functions in mammals. Here, we used ELOVL2-ablated (Elovl2(-/-)) mice to show that the PUFAs with 24-30 carbon atoms of the ω-6 family in testis are indispensable for normal sperm formation and fertility in male mice. The lack of Elovl2 was associated with a complete arrest of spermatogenesis, with seminiferous tubules displaying only spermatogonia and primary spermatocytes without further germinal cells. Furthermore, based on acyl-CoA profiling, heterozygous Elovl2(+/-) male mice exhibited haploinsufficiency, with reduced levels of C28:5 and C30:5n-6 PUFAs, which gave rise to impaired formation and function of haploid spermatides. These new insights reveal a novel mechanism involving ELOVL2-derived PUFAs in mammals and previously unrecognized roles for C28 and C30 n-6 PUFAs in male fertility. In accordance with the function suggested for ELOVL2, the Elovl2(-/-) mice show distorted levels of serum C20 and C22 PUFAs from both the n-3 and the n-6 series. However, dietary supplementation with C22:6n-3 could not restore male fertility to Elovl2(+/-) mice, suggesting that the changes in n-6 fatty acid composition seen in the testis of the Elovl2(+/-) mice, cannot be compensated by increased C22:6n-3 content.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- fatty acid elongase
- omega-6
- spermatogenesis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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