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Tracking Ca2+ ATPas...
Tracking Ca2+ ATPase intermediates in real time by x-ray solution scattering
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- Ravishankar, Harsha (author)
- Umeå universitet,Kemiska institutionen
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Pedersen, Martin Nors (author)
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Eklund, Mattias (author)
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Sitsel, Aljona (author)
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- Li, Chenge (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Duelli, Annette (author)
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Levantino, Matteo (author)
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Wulff, Michael (author)
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- Barth, Andreas (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Olesen, Claus (author)
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Nissen, Poul (author)
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- Andersson, Magnus (author)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- American Association for the Advancement of Science, 2020
- 2020
- English.
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In: Science Advances. - : American Association for the Advancement of Science. - 2375-2548. ; 6:12
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) transporters regulate calcium signaling by active calcium ion reuptake to internal stores. Structural transitions associated with transport have been characterized by x-ray crystallography, but critical intermediates involved in the accessibility switch across the membrane are missing. We combined time-resolved x-ray solution scattering (TR-XSS) experiments and molecular dynamics (MD) simulations for real-time tracking of concerted SERCA reaction cycle dynamics in the native membrane. The equilibrium [Ca-2] E1 state before laser activation differed in the domain arrangement compared with crystal structures, and following laser-induced release of caged ATP, a 1.5-ms intermediate was formed that showed closure of the cytoplasmic domains typical of E1 states with bound Ca2+ and ATP. A subsequent 13-ms transient state showed a previously unresolved actuator (A) domain arrangement that exposed the ADP-binding site after phosphorylation. Hence, the obtained TR-XSS models determine the relative timing of so-far elusive domain rearrangements in a native environment.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ravishankar, Har ...
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Pedersen, Martin ...
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Eklund, Mattias
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Sitsel, Aljona
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Li, Chenge
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Duelli, Annette
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show more...
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Levantino, Matte ...
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Wulff, Michael
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Barth, Andreas
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Olesen, Claus
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Nissen, Poul
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Andersson, Magnu ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Science Advances
- By the university
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Umeå University
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Stockholm University