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Nanodelivery of Cer...
Nanodelivery of Cerebrolysin and Rearing in Enriched Environment Induce Neuroprotective Effects in a Preclinical Rat Model of Parkinson's Disease
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- Requejo, C. (författare)
- Univ Basque Country UPV EHU, Dept Neurosci, LaNCE, Leioa, Vizcaya, Spain.
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- Ruiz-Ortega, J. A. (författare)
- Univ Basque Country UPV EHU, Dept Pharmacol, Leioa, Vizcaya, Spain.
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- Cepeda, H. (författare)
- Univ Basque Country UPV EHU, Dept Neurosci, LaNCE, Leioa, Vizcaya, Spain.
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- Sharma, A. (författare)
- Uppsala Univ, Int Expt CNS Injury & Repair, Uppsala, Sweden.
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- Sharma, H.S. (författare)
- Uppsala universitet,Anestesiologi och intensivvård,Univ Basque Country UPV EHU, Dept Neurosci, LaNCE, Leioa, Vizcaya, Spain.;Uppsala Univ, Int Expt CNS Injury & Repair, Uppsala, Sweden.
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- Ozkizilcik, A. (författare)
- Univ Arkansas, Dept Biomed Engn, Fayetteville, AR 72701 USA.
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- Tian, R. (författare)
- Univ Arkansas, Dept Biomed Engn, Fayetteville, AR 72701 USA.;Univ Arkansas, Dept Chem & Biochem, Fayetteville, AR 72701 USA.
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- Moessler, H. (författare)
- Ever Neuro Pharma, Drug Discovery & Develop, Oberburgau, Austria.
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- Ugedo, L. (författare)
- Univ Basque Country UPV EHU, Dept Pharmacol, Leioa, Vizcaya, Spain.
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- Lafuente, J. V. (författare)
- Univ Basque Country UPV EHU, Dept Neurosci, LaNCE, Leioa, Vizcaya, Spain.;BioCruces Hlth Res Inst, Nanoneurosurg Grp, Baracaldo 48903, Vizcaya, Spain.;Univ Autonoma Chile, Fac Hlth Sci, Santiago, Chile.
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Univ Basque Country UPV EHU, Dept Neurosci, LaNCE, Leioa, Vizcaya, Spain Univ Basque Country UPV EHU, Dept Pharmacol, Leioa, Vizcaya, Spain. (creator_code:org_t)
- 2017-08-24
- 2018
- Engelska.
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Ingår i: Molecular Neurobiology. - : Humana Press. - 0893-7648 .- 1559-1182. ; 55:1, s. 286-299
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Rearing in enriched environment (EE) improves the recuperation in animal models of Parkinson's disease (PD). Administration of TiO2-nanowired cerebrolysin (CBL) could represent an additional strategy to protect or repair the nigrostriatal system. This study aims to explore morphofunctional and biochemical changes in a preclinical stage of PD testing the synergistic efficiency of combining both strategies, housing in EE, and nanodelivery of CBL. Sprague-Dawley male rats receiving intrastriatally 6-hydroxydopamine after a short evolution time were segregated into CBL group (rats receiving nanowired CBL), EE group (rats housed in EE), CBL + EE group (rats housed in EE and receiving nanowired CBL), and control group (rats without additional treatment). Prodromic stage and treatment effects were characterized by the presence of motor symptoms (amphetamine-induced rotational behavior test). Tyrosine hydroxylase (TH) immunohistochemistry and Western blot (p-Akt/Akt and p-ERK/ERK 1/2 as survival markers and caspase-3 as apoptotic marker) were performed in striatum and SN. A decrease in motor symptoms was shown by rats receiving CBL. EE monitoring cages revealed that rats from CBL + EE group showed more significant number of laps in the wheel than EE group. In SN, CBL + EE group also presented the highest neuronal density. Moreover, p-Akt/Akt and p-ERK/ERK 1/2 ratio was significant higher and caspase-3 expression was lower in CBL + EE group. In conclusion, the combination of CBL and EE provided evidence of neuoprotective-neurorestorative mechanisms by which this combined strategy promoted morphofunctional improvement by activation of survival pathways after dopamine depletion in a preclinical model of PD.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Parkinson'sdisease
- 6-OHDA
- Preclinical stage
- Nanowired cerebrolysin
- Enriched environment
- Neuroprotection
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Requejo, C.
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Ruiz-Ortega, J. ...
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Cepeda, H.
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Sharma, A.
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Sharma, H.S.
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Ozkizilcik, A.
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visa fler...
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Tian, R.
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Moessler, H.
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Ugedo, L.
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Lafuente, J. V.
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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Molecular Neurob ...
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Uppsala universitet