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Which methods are a...
Which methods are appropriate for the detection of Staphylococcus argenteus and is it worthwhile to distinguish S. argenteus from S. aureus?
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- Kaden, Rene, 1975- (författare)
- Uppsala universitet,Klinisk mikrobiologi
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- Engstrand, Lars (författare)
- Karolinska Institutet,Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Solna, Sweden
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- Rautelin, Hilpi (författare)
- Uppsala universitet,Klinisk mikrobiologi
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- Johansson, Cecilia (författare)
- Uppsala universitet,Klinisk mikrobiologi
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(creator_code:org_t)
- 2018
- 2018
- Engelska.
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Ingår i: Infection and Drug Resistance. - 1178-6973. ; 11, s. 2335-2344
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.2...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Purpose: To further analyze a clinical isolate originally identified as methicillin-resistant Staphylococcus aureus (MRSA) using whole-genome sequencing and comparative genomics.Materials and methods: Classical diagnostic methods such as cultivation, biochemical tests, and PCR were supplemented with whole-genome sequencing and comparative genomics, to identify the isolate.Results: The isolate was phenotypically similar to MRSA. However, the presence of the nuc gene could not be confirmed using PCR, while it was positive for the mecA gene. Whole-genome sequencing correctly identified the isolate as Staphylococcus argenteus. The isolate possessed several resistance genes, such as mecA, blaZ (beta-lactam antibiotics) and dfrG (trimethoprim). The me gene differed from that of MRSA. Six phylogenetic distinct clusters were identified by average nucleotide identity (ANI) analysis of all available S. argenteus whole-genome sequences. Our isolate, RK308, clustered with those isolated in Europe and Asia.Conclusion: Due to the invasive potential, the multi-drug resistance and the similarity to MRSA, S. argenteus should be included in the MRSA screening. Due to the divergent genome compared to MRSA, new PCR approaches have to be developed to avoid an unnoticed spreading of S. argenteus.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Staphylococcus argenteus
- Staphylococcus aureus
- MRSA
- whole-genome sequencing
- clinical diagnostics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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