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Purification of the...
Purification of the isoflavonoid puerarin by adsorption chromatography on cross-linked 12% agarose
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He,, X. (författare)
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T., Tan (författare)
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- Janson, J.-C. (författare)
- Uppsala universitet,Institutionen för ytbioteknik med Centrum för ytbioteknik,Institutionen för fysikalisk och analytisk kemi
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: J. Chromatogr. A. ; 1057:1-2, s. 95-100
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- The isoflavonoid puerarin in extracts of the well-known traditional Chinese drug Radix puerariae (root of the plant Pueraria lobata) can be separated from other isoflavonoids by adsorption chromatography on the cross-linked 12% agarose gel Superose 12 equilibrated in distilled water. The adsorption is totally quenched by the addition of 50% acetic acid. The separation of the isoflavonoids is tentatively ascribed to interaction with the residues of the cross-linking reagents used in the manufacturing process of Superose 12. Thus, no useful separation can be achieved with non-cross-linked 12% agarose gel media. Symmetric elution profiles at high sample loadings (16 mg on a 24 ml column) suggest linear adsorption isotherms for the isoflavonoids.
Nyckelord
- Isoflavonoid separation
- Agarose cross-linker
- Puerarin
- Acetic acid quenching
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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