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BAFF, stimulatory D...
BAFF, stimulatory DNA and IL-15 stimulates IgA(+) memory B cells and provides a novel approach for analysis of memory responses to mucosal vaccines.
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- Tengvall, Sara, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Lundgren, Anna, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Quiding-Järbrink, Marianne, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Svennerholm, Ann-Mari, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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(creator_code:org_t)
- Elsevier BV, 2010
- 2010
- English.
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In: Vaccine. - : Elsevier BV. - 1873-2518 .- 0264-410X. ; 28:33, s. 5445-50
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Abstract
Subject headings
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- Assessment of immune responses induced by mucosal vaccines is to a large extent based on measurement of IgA levels in mucosal secretions and detection of short-lived effector IgA-secreting cells circulating in peripheral blood. Since these immunological parameters poorly reflect long-term IgA-mediated responses, we sought to investigate novel approaches that would enable detection of vaccine specific IgA memory B cells. We demonstrate that stimulation of human peripheral blood mononuclear cells in vitro with immunostimulatory DNA in combination with B cell-activating factor (BAFF) and IL-15 promotes differentiation of IgA memory B cells to IgA-secreting cells. By using the inactivated oral cholera vaccine Dukoral we demonstrate that vaccine specific IgA memory B cells are induced by oral immunization and are circulating for at least 9 months after vaccination. We also show that stimulated IgA memory B cells do not secrete IgA unless they reencounter the specific antigen.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Adjuvants
- Immunologic
- pharmacology
- Adult
- Antibodies
- Bacterial
- blood
- immunology
- B-Cell Activating Factor
- immunology
- pharmacology
- B-Lymphocytes
- immunology
- Cholera Vaccines
- immunology
- pharmacology
- DNA
- immunology
- pharmacology
- Female
- Humans
- Immunity
- Mucosal
- drug effects
- immunology
- Immunization
- methods
- Immunoglobulin A
- blood
- immunology
- Immunologic Memory
- drug effects
- immunology
- Interleukin-15
- immunology
- pharmacology
- Male
- Time Factors
- Vaccines
- Inactivated
- immunology
- pharmacology
Publication and Content Type
- ref (subject category)
- art (subject category)
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