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An in situ fracture...
An in situ fracture device to image lipids in single cells using ToF-SIMS
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- Lanekoff, Ingela, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Kurczy, Michael, 1980 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Adams, Kelly L. (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Malm, J (författare)
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- Karlsson, Roger, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Sjovall, P (författare)
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- Ewing, Andrew G, 1957 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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(creator_code:org_t)
- 2010-06-22
- 2011
- Engelska.
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Ingår i: SURFACE AND INTERFACE ANALYSIS. - : Wiley. - 0142-2421 .- 1096-9918. ; 43:1-2, s. 257-260
- Relaterad länk:
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) imaging yields molecule-specific images showing the spatial distribution of specific lipids with submicron resolution, making it a potentially powerful method for studying a variety of biological questions in single cells. In order to make possible the analysis of hydrated cells in vacuum, we have designed a device for in situ freeze-fracture of cell samples. PC12 cells are frozen between two silicon shards in a sandwich geometry, and the fracture is conducted under vacuum inside the analysis chamber of the instrument. After freeze-fracture, ToF-SIMS analysis was carried out using a Bi cluster ion source, providing high-resolution images of single cells. We also show that when combining freeze-fracture and cluster SIMS imaging, low abundant biologically important lipids, specifically PE, PC and non-SM PC, can be imaged in single cells. Finally, K+ ion localization was used as a diagnostic to identify fracture planes through the cell for these fractured samples.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- tof-sims imaging
- pc12 cells
- freeze-fracture
- phospholipids
- pc12 cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Lanekoff, Ingela ...
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Kurczy, Michael, ...
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Adams, Kelly L.
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Malm, J
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Karlsson, Roger, ...
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Sjovall, P
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visa fler...
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Ewing, Andrew G, ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
- Artiklar i publikationen
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SURFACE AND INTE ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola
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Uppsala universitet