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Isolation and parti...
Isolation and partial characterization of blood group A and H active glycosphingolipids of rat small intestine.
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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- Hansson, Gunnar C., 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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Karlsson, K A (författare)
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visa fler...
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Leffler, H (författare)
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visa färre...
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(creator_code:org_t)
- 1982
- 1982
- Engelska.
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Ingår i: The Journal of biological chemistry. - 0021-9258. ; 257:2, s. 906-12
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Blood group A and H active glycosphingolipids have been isolated from rat small intestine. By mass spectrometry of the permethylated and LiAlH4-reduced permethylated glycolipid derivatives, the A glycolipids were shown to contain four (A-4), six (A-6), and 12 (A-12) sugar residues, respectively. The anomeric structure of the A-4 and A-6 glycolipids was established by proton NMR spectroscopy of the permethylated-reduced derivatives. Acid degradation and gas chromatography were used for analysis of binding positions. The structures of the A-4 and A-6 glycolipids were GalNAcp alpha 1 leads to 3Galp(2 comes from 1Fucp alpha) beta 1 leads to Glcp beta 1 leads to 1Cer and GalNAcp alpha 1 leads to 3Galp(2 comes from 1Fucp alpha) beta 1 leads to 3GlcNAcp beta 1 leads to 4Galp beta 1 leads to 4Glcp beta 1 leads to 1Cer. The third glycolipid (A-12) was a branched dodecaglycosylceramide with two blood group A determinants. The complete structure of this glycolipid has not yet been solved. The blood group A activity was the same for the A-6 and A-12 glycolipids based on an equal number of blood group A determinants, but the activity of the A-4 compound was only about half of the others. The A-6 glycolipid was based on a type 1 (Gal beta 1 leads to 3GlcNAc) carbohydrate chain, thus differing from the already known isomer based on a type 2 chain (Gal beta 1 leads to 4GlcNAc) present in human erythrocyte. The blood group A activity of these two glycolipids was found to be identical. The three rat intestinal blood group A active glycolipids were exclusively located to the mucosa epithelial cells. The blood group H active tri- and pentaglycosylceramides (H-3 and H-5), presumed to be the precursors of the A-4 and A-6 glycolipids, were also identified. A 10-sugar glycolipid (H-10), a possible precursor of A-12, was not detected.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- ABO Blood-Group System
- Animals
- Carbohydrate Conformation
- Carbohydrate Sequence
- Glycosphingolipids
- isolation & purification
- Intestine
- Small
- analysis
- Magnetic Resonance Spectroscopy
- Male
- Mass Spectrometry
- Rats
- Rh-Hr Blood-Group System
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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