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Characterizing the ...
Characterizing the Catecholamine Content of Single Mammalian Vesicles by Collision-Adsorption Events at an Electrode
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- Dunevall, Johan, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Mashadi Fathali, Hoda, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Najafinobar, Neda, 1985 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Lovric, Jelena, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wigström, Joakim, 1971 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Cans, Ann-Sofie, 1971 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ewing, Andrew G, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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(creator_code:org_t)
- 2015-03-31
- 2015
- English.
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In: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 137:13, s. 4344-4346
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Abstract
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- We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 mu m diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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Dunevall, Johan, ...
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Mashadi Fathali, ...
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Najafinobar, Ned ...
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Lovric, Jelena, ...
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Wigström, Joakim ...
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Cans, Ann-Sofie, ...
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show more...
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Ewing, Andrew G, ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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Journal of the A ...
- By the university
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University of Gothenburg
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Chalmers University of Technology