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Different protein A...
Different protein A immunosorbents may have different binding specificity for rat immunoglobulins
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- Nilsson, Rune (author)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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Myhre, E (author)
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Kronvall, G (author)
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Sjögren, H O (author)
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(creator_code:org_t)
- 1983
- 1983
- English.
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In: Journal of Immunological Methods. - 1872-7905. ; 62:2, s. 241-245
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower uptake of these subclasses. No differences were seen for IgG2a and IgG2c. Protein A on different solid phases cannot be used interchangeably without confirmation of binding specificity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- protein A-Sepharose
- protein A macrobeads
- rat IgG subclasses
- immunosorbents
Publication and Content Type
- art (subject category)
- ref (subject category)
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