Search: id:"swepub:oai:lup.lub.lu.se:33af3992-3c94-452e-ad55-e5d571b164d3" >
Effects of fat, bee...
Effects of fat, beef and fiber in diets on activities of sphingomyelinase, ceramidase and caspase-3 in rat colonic mucosa
-
- Yang, Liping (author)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine
-
Mutanen, M (author)
-
- Cheng, Yajun (author)
- Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine
-
show more...
-
- Duan, Rui-Dong (author)
- Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine
-
show less...
-
(creator_code:org_t)
- 2002-08-02
- 2002
- English.
-
In: Medical Principles and Practice. - : S. Karger AG. - 1011-7571 .- 1423-0151. ; 11:3, s. 150-156
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://www.karger.c...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Objective: The present study investigates the activity changes of sphingomyelinase (SMase), ceramidase and caspase-3 in colonic mucosa of rats induced by high fat and red meat diets. Method. For a period of 3 weeks, rats were fed protein without fat (control), high fat only, high fat with beef, and high fat with fiber (cellulose) diets. The fat content (22.4%) was constant in the three high fat diets. Then, the colonic mucosae were scraped and homogenized and the activities of SMase, ceramidase and caspase-3 determined. Results: Compared to the control diet, the fat diet and fat with beef diet reduced intestinal alkaline SMase by 80 and 84%, respectively; ceramidase activity by 60 and 92%, respectively, and caspase-3 activity by 40 and 75%, respectively. The activities of acid and neutral SMases were also decreased by fat and fat with beef diets but to a smaller extent than those of alkaline SMase. Supplement of fiber in the fat diet had no effect on the changes of alkaline SMase activity but prevented fat-induced decreases in acid and neutral SMase activities and partially prevented those of ceramidase and caspase-3 activities. The activity of intestinal alkaline phosphatase was not changed by any of the diets. Conclusion: Fat, beef and fiber significantly affect the enzymes responsible for sphingomyelin metabolism and apoptosis in the colon. The effects may have implications in colonic tumorigenesis related to dietary factors.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
Keyword
- fiber
- red meat
- fat
- ceramidase
- sphingomyelinase
- caspase-3
- colon
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database