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Characterization of...
Characterization of the genome, proteome, and structure of yersiniophage φR1-37
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- Skurnik, Mikael (författare)
- University of Helsinki Haartman Institute,Helsinki University Central Hospital
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- Hyytiäinen, Heidi J. (författare)
- University of Helsinki,University of Helsinki Haartman Institute
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- Happonen, Lotta J. (författare)
- Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Molekylär patogenes,Forskargrupper vid Lunds universitet,BioMS,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Molecular Pathogenesis,Lund University Research Groups,University of Helsinki
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- Kiljunen, Saija (författare)
- University of Turku
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- Datta, Neeta (författare)
- University of Helsinki Haartman Institute
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- Mattinen, Laura (författare)
- University of Helsinki Haartman Institute
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- Williamson, Kirsty (författare)
- University of Helsinki
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- Kristo, Paula (författare)
- University of Helsinki Haartman Institute
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- Szeliga, Magdalena (författare)
- University of Helsinki Haartman Institute
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- Kalin-Mänttäri, Laura (författare)
- University of Helsinki Haartman Institute
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- Ahola-Iivarinen, Elina (författare)
- University of Helsinki
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- Kalkkinen, Nisse (författare)
- University of Helsinki
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- Butcher, Sarah J. (författare)
- University of Helsinki
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(creator_code:org_t)
- 2012
- 2012
- Engelska 18 s.
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Ingår i: Journal of Virology. - 0022-538X. ; 86:23, s. 12625-12642
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes scattered throughout the genome. A total of 269 of the ORFs (73%) lack homologues in sequence databases. Based on terminator and promoter sequences identified from the intergenic regions, the phage genome was predicted to consist of 40 to 60 transcriptional units. Image reconstruction revealed that the φR1-37 capsid consists of hexameric capsomers arranged on a T=27 lattice similar to the bacteriophage φKZ. The tail of φR1-37 has a contractile sheath. We conclude that phage φR1-37 is a representative of a novel phage type that carries the dU-containing genome in a φKZ-like head.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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Skurnik, Mikael
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Hyytiäinen, Heid ...
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Happonen, Lotta ...
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Kiljunen, Saija
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Datta, Neeta
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Mattinen, Laura
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Williamson, Kirs ...
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Kristo, Paula
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Szeliga, Magdale ...
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Kalin-Mänttäri, ...
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Ahola-Iivarinen, ...
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Kalkkinen, Nisse
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Butcher, Sarah J ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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Lunds universitet