Photobleaching-Insensitive Fluorescence Diagnostics in Skin and Brain Tissue

• In this paper, we investigate the possibility of using accurate prediction models for the prediction of protoporphyrin bleaching dynamics to achieve photobleaching-insensitive methods to improve the evaluation of data in an existing clinical fluorescence-guided resection technique. To simulate the scenario, measurements were carried out in vivo on skin of healthy volunteers using a compact fiber-based fluorescence spectroscopy system. We have developed an effective method for the parameterization of sequences of bleaching spectra. We analyze convergence and decay rates with respect to initial conditions and excitation irradiance. We also discuss the consequences and the potential for bleaching-insensitive measurements and their applicability in a few examples from in vivo open brain surgery.

Ämnesord

NATURVETENSKAP  -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)

NATURAL SCIENCES  -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)

Nyckelord

Fluorescence-guided resection

FGR

5-ALA

PpIX

protoporphyrin

photobleaching

laser-induced fluorescence spectroscopy

LIF

optical

diagnostics

tissue optics

skin

brain

dynamic models

system

identification

state space models

Fluorescence-guided resection

TECHNOLOGY

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)