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Investigation of Ce...
Investigation of Cerebral Mitochondrial Injury in a Porcine Survivor Model of Carbon Monoxide Poisoning
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- Mavroudis, Constantine D. (författare)
- The Children's Hospital of Philadelphia
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- Lewis, Alistair (författare)
- The Children's Hospital of Philadelphia,University of Pennsylvania
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- Greenwood, John C. (författare)
- University of Pennsylvania
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- Kelly, Matthew (författare)
- University of Pennsylvania,The Children's Hospital of Philadelphia
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- Ko, Tiffany S. (författare)
- The Children's Hospital of Philadelphia
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- Forti, Rodrigo M. (författare)
- The Children's Hospital of Philadelphia
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- Shin, Samuel S. (författare)
- University of Pennsylvania
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- Shofer, Frances S. (författare)
- University of Pennsylvania
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- Ehinger, Johannes K. (författare)
- Lund University,Lunds universitet,Öron-, näs- och halssjukdomar, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Mitokondriell Medicin,Forskargrupper vid Lunds universitet,Otorhinolaryngology (Lund),Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Mitochondrial Medicine,Lund University Research Groups,Skåne University Hospital
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- Baker, Wesley B. (författare)
- The Children's Hospital of Philadelphia
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- Kilbaugh, Todd J. (författare)
- The Children's Hospital of Philadelphia
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- Jang, David H. (författare)
- The Children's Hospital of Philadelphia,University of Pennsylvania
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(creator_code:org_t)
- 2024
- 2024
- Engelska.
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Ingår i: Journal of Medical Toxicology. - 1556-9039. ; 20:1, s. 39-48
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Introduction: Carbon monoxide (CO) is a colorless and odorless gas that is a leading cause of environmental poisoning in the USA with substantial mortality and morbidity. The mechanism of CO poisoning is complex and includes hypoxia, inflammation, and leukocyte sequestration in brain microvessel segments leading to increased reactive oxygen species. Another important pathway is the effects of CO on the mitochondria, specifically at cytochrome c oxidase, also known as Complex IV (CIV). One of the glaring gaps is the lack of rigorous experimental models that may recapitulate survivors of acute CO poisoning in the early phase. The primary objective of this preliminary study is to use our advanced swine platform of acute CO poisoning to develop a clinically relevant survivor model to perform behavioral assessment and MRI imaging that will allow future development of biomarkers and therapeutics. Methods: Four swine (10 kg) were divided into two groups: control (n = 2) and CO (n = 2). The CO group received CO at 2000 ppm for over 120 min followed by 30 min of re-oxygenation at room air for one swine and 150 min followed by 30 min of re-oxygenation for another swine. The two swine in the sham group received room air for 150 min. Cerebral microdialysis was performed to obtain semi real-time measurements of cerebral metabolic status. Following exposures, all surviving animals were observed for a 24-h period with neurobehavioral assessment and imaging. At the end of the 24-h period, fresh brain tissue (cortical and hippocampal) was immediately harvested to measure mitochondrial respiration. Results: While a preliminary ongoing study, animals in the CO group showed alterations in cerebral metabolism and cellular function in the acute exposure phase with possible sustained mitochondrial changes 24 h after the CO exposure ended. Conclusions: This preliminary research further establishes a large animal swine model investigating survivors of CO poisoning to measure translational metrics relevant to clinical medicine that includes a basic neurobehavioral assessment and post exposure cellular measures.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Basic science
- Biomarker
- Carbon monoxide
- Mitochondria
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Mavroudis, Const ...
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Lewis, Alistair
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Greenwood, John ...
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Kelly, Matthew
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Ko, Tiffany S.
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Forti, Rodrigo M ...
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visa fler...
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Shin, Samuel S.
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Shofer, Frances ...
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Ehinger, Johanne ...
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Baker, Wesley B.
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Kilbaugh, Todd J ...
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Jang, David H.
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
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Journal of Medic ...
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Lunds universitet