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Genome-wide discove...
Genome-wide discovery and verification of novel structured RNAs in Plasmodium falciparum
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- Mourier, Tobias (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
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Carret, Celine (author)
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Kyes, Sue (author)
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Christodoulou, Zoe (author)
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Gardner, Paul P. (author)
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Jeffares, Daniel C. (author)
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Pinches, Robert (author)
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Barrell, Bart (author)
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Berriman, Matt (author)
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Griffiths-Jones, Sam (author)
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Ivens, Alasdair (author)
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Newbold, Chris (author)
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Pain, Arnab (author)
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(creator_code:org_t)
- 2007-12-20
- 2008
- English.
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In: Genome Research. - : Cold Spring Harbor Laboratory. - 1549-5469 .- 1088-9051. ; 18:2, s. 281-292
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http://genome.cshlp....
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Abstract
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- We undertook a genome-wide search for novel noncoding RNAs (ncRNA) in the malaria parasite Plasmodium falciparum. We used the RNAz program to predict structures in the noncoding regions of the P. falciparum 3D7 genome that were conserved with at least one of seven other Plasmodium spp. genome sequences. By using Northern blot analysis for 76 high-scoring predictions and microarray analysis for the majority of candidates, we have verified the expression of 33 novel ncRNA transcripts including four members of a ncRNA family in the asexual blood stage. These transcripts represent novel structured ncRNAs in P. falciparum and are not represented in any RNA databases. We provide supporting evidence for purifying selection acting on the experimentally verified ncRNAs by comparing the nucleotide substitutions in the predicted ncRNA candidate structures in P. falciparum with the closely related chimp malaria parasite P. reichenowi. The high confirmation rate within a single parasite life cycle stage suggests that many more of the predictions may be expressed in other stages of the organism's life cycle.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Mourier, Tobias
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Carret, Celine
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Kyes, Sue
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Christodoulou, Z ...
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Gardner, Paul P.
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Jeffares, Daniel ...
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show more...
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Pinches, Robert
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Barrell, Bart
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Berriman, Matt
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Griffiths-Jones, ...
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Ivens, Alasdair
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Newbold, Chris
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Pain, Arnab
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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Genome Research
- By the university
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Lund University