Search: id:"swepub:oai:prod.swepub.kib.ki.se:146153647" >
Cartilage Oligomeri...
Cartilage Oligomeric Matrix Protein Induced Arthritis-A New Model for Rheumatoid Arthritis in the C57BL/6 Mouse
-
Zhao, YJ (author)
-
- Urbonaviciute, V (author)
- Karolinska Institutet
-
- Xu, BZ (author)
- Karolinska Institutet
-
show more...
-
Cai, WW (author)
-
Sener, Z (author)
-
- Ge, CR (author)
- Karolinska Institutet
-
- Holmdahl, R (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- 2021-02-23
- 2021
- English.
-
In: Frontiers in immunology. - : Frontiers Media SA. - 1664-3224. ; 12, s. 631249-
- Related links:
-
https://www.frontier...
-
show more...
-
http://kipublication...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- The most commonly used strains in experimental research, including genetically modified strains, are C57BL/6 mice. However, so far, no reliable model for rheumatoid arthritis is available, mainly due to the restriction by the MHC class II haplotype H-2b. Collagen-induced arthritis (CIA) is the most widely used animal model of rheumatoid arthritis, but C57BL/6 strain is resistant to CIA because there is no collagen II peptide associated with H-2b. To establish a rheumatoid arthritis model in C57BL/6 mice, we immunized C57BL/6NJ (B6N) mice with human cartilage oligomeric matrix protein (COMP), which induced severe arthritis with high incidence, accompanied by a strong auto-antibody response. Native COMP was required, as denatured COMP lost its ability to induce arthritis in B6N mice. An immunodominant COMP peptide was identified as the key T cell epitope, with a perfect fit into the Ab class II peptide binding pocket. A critical amino acid in this peptide was found to be phenylalanine at position 95. Recombinant COMP mutated at position 95 (COMP_F95S) lost its ability to induce arthritis or a strong immune response in the B6N mice. In conclusion, A new model for RA has been established using C57BL/6 mice through immunization with COMP, which is dependent on a COMP specific peptide binding Ab, thus in similarity with CIA in Aq expressing strains.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database