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A microfluidic pers...
A microfluidic perspective on conventional in vitro transcranial direct current stimulation methods
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- Lu, Han (author)
- Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; BrainLinks-BrainTools Center, University of Freiburg, Freiburg, Germany,Albert-Ludwigs-Universität Freiburg,University of Freiburg
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- Shaner, Sebastian (author)
- BrainLinks-BrainTools Center, University of Freiburg, Freiburg, Germany; Department of Microsystems Engineering (IMTEK), University of Freiburg, Freiburg, Germany,Albert-Ludwigs-Universität Freiburg,University of Freiburg
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- Otte, Elisabeth (author)
- Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; BrainLinks-BrainTools Center, University of Freiburg, Freiburg, Germany; Department of Microsystems Engineering (IMTEK), University of Freiburg, Freiburg, Germany,Albert-Ludwigs-Universität Freiburg,University of Freiburg
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- Asplund, Maria (author)
- Luleå tekniska universitet,Omvårdnad och medicinsk teknik,BrainLinks-BrainTools Center, University of Freiburg, Freiburg, Germany; Department of Microsystems Engineering (IMTEK), University of Freiburg, Freiburg, Germany; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Freiburg, Germany; Department of Microtechnology and Nanoscience, Chalmers University of Technology, Gothenburg, Sweden,Albert-Ludwigs-Universität Freiburg,University of Freiburg,Luleå tekniska universitet (LTU),Luleå University of Technology (LTU),Chalmers tekniska högskola,Chalmers University of Technology
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- Vlachos, Andreas (author)
- Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany; BrainLinks-BrainTools Center, University of Freiburg, Freiburg, Germany; Center for Basics in Neuromodulation (NeuroModulBasics), Faculty of Medicine, University of Freiburg, Freiburg, Germany,Albert-Ludwigs-Universität Freiburg,University of Freiburg
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(creator_code:org_t)
- Elsevier B.V. 2023
- 2023
- English.
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In: Journal of Neuroscience Methods. - : Elsevier B.V.. - 0165-0270 .- 1872-678X. ; 385
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Abstract
Subject headings
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- Transcranial direct current stimulation (tDCS) is a promising non-invasive brain stimulation method to treat neurological and psychiatric diseases. However, its underlying neural mechanisms warrant further investigation. Indeed, dose–response interrelations are poorly understood. Placing explanted brain tissue, mostly from mice or rats, into a uniform direct current electric field (dcEF) is a well-established in vitro system to elucidate the neural mechanism of tDCS. Nevertheless, we will show that generating a defined, uniform, and constant dcEF throughout a brain slice is challenging. This article critically reviews the methods used to generate and calibrate a uniform dcEF. We use finite element analysis (FEA) to evaluate the widely used parallel electrode configuration and show that it may not reliably generate uniform dcEF within a brain slice inside an open interface or submerged chamber. Moreover, equivalent circuit analysis and measurements inside a testing chamber suggest that calibrating the dcEF intensity with two recording electrodes can inaccurately capture the true EF magnitude in the targeted tissue when specific criteria are not met. Finally, we outline why microfluidic chambers are an effective and calibration-free approach of generating spatiotemporally uniform dcEF for DCS in vitro studies, facilitating accurate and fine-scale dcEF adjustments. We are convinced that improving the precision and addressing the limitations of current experimental platforms will substantially improve the reproducibility of in vitro experimental results. A better mechanistic understanding of dose–response relations will ultimately facilitate more effective non-invasive stimulation therapies in patients.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Annan medicinteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Other Medical Engineering (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- direct current electric field
- electrotaxis
- finite element analysis
- in vitro
- transcranial direct current stimulation
- Medical Engineering
- Medicinsk teknik
Publication and Content Type
- ref (subject category)
- for (subject category)
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