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Shared Molecular Ta...
Shared Molecular Targets Confer Resistance over Short and Long Evolutionary Timescales
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- Li, Jing (author)
- Université Côte d'Azur,University of Côte d'Azur
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- Vázquez-García, Ignacio (author)
- Columbia University in the City of New York,University Of Cambridge
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- Persson, Karl, 1988 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- González, Asier (author)
- Universität Basel,University of Basel
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- Yue, Jia Xing (author)
- Université Côte d'Azur,University of Côte d'Azur
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- Barré, Benjamin P. (author)
- Université Côte d'Azur,University of Côte d'Azur
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- Hall, Michael N. (author)
- Universität Basel,University of Basel
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- Long, Anthony (author)
- University of California at Irvine (UCI)
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- Warringer, Jonas, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Mustonen, Ville (author)
- Helsingin Yliopisto,University of Helsinki
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- Liti, Gianni (author)
- Université Côte d'Azur,University of Côte d'Azur
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(creator_code:org_t)
- 2019-01-17
- 2019
- English.
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In: Molecular Biology and Evolution. - : Oxford University Press (OUP). - 1537-1719 .- 0737-4038. ; 36:4, s. 691-708
- Related links:
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Abstract
Subject headings
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- Pre-existing and de novo genetic variants can both drive adaptation to environmental changes, but their relative contributions and interplay remain poorly understood. Here we investigated the evolutionary dynamics in drug-treated yeast populations with different levels of pre-existing variation by experimental evolution coupled with time-resolved sequencing and phenotyping. We found a doubling of pre-existing variation alone boosts the adaptation by 64.1% and 51.5% in hydroxyurea and rapamycin, respectively. The causative pre-existing and de novo variants were selected on shared targets: RNR4 in hydroxyurea and TOR1, TOR2 in rapamycin. Interestingly, the pre-existing and de novo TOR variants map to different functional domains and act via distinct mechanisms. The pre-existing TOR variants from two domesticated strains exhibited opposite rapamycin resistance effects, reflecting lineage-specific functional divergence. This study provides a dynamic view on how pre-existing and de novo variants interactively drive adaptation and deepens our understanding of clonally evolving populations.
Subject headings
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- de novo mutation
- adaptation
- budding yeast
- drug resistance
- pre-existing genetic variation
- adaptation
- budding yeast
- de novo mutation
- drug resistance
- pre-existing genetic variation
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Li, Jing
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Vázquez-García, ...
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Persson, Karl, 1 ...
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González, Asier
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Yue, Jia Xing
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Barré, Benjamin ...
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show more...
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Hall, Michael N.
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Long, Anthony
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Warringer, Jonas ...
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Mustonen, Ville
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Liti, Gianni
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Evolutionary Bio ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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Molecular Biolog ...
- By the university
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Chalmers University of Technology
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University of Gothenburg