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Redesign and recons...
Redesign and reconstruction of a mevalonate pathway and its application in terpene production in Escherichia coli
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- Choi, BoHyun, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kim, JinHo, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Choi, Sun Young (author)
- Ajou University
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- Han, Se Jong (author)
- Korea Polar Research Institute
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- Lee, Pyung Cheon (author)
- Ajou University
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
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In: Bioresource Technology Reports. - : Elsevier BV. - 2589-014X. ; 7
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https://research.cha... (primary) (free)
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https://doi.org/10.1...
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Abstract
Subject headings
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- Metabolic engineering of heterologous biosynthetic pathways in microbial hosts is an alternative way for producing high-value biochemicals. Here, heterologous mevalonate (MVA) and prenyl pyrophosphate (IPP) pathways of a new isolate Enterococcus kingsejongensis Gf1 were redesigned and reconstructed in Escherichia coli. Two MVA upstream enzymes of E. kingsejongensis Gf1 were functionally expressed and overexpression of the two enzymes led to a yield of 52.5 g/L MVA after 65 h of fed-batch fermentation. Next, the functions of three MVA downstream enzymes were verified by addition of exogenous MVA. Finally, whole MVA pathway enzymes and IPP pathway enzymes were combined in E. coli in order to overproduce squalene and β-carotene. The E. coli strains coexpressing entire MVA and IPP pathway enzymes enhanced squalene production by 15-fold and β-carotene production by 2.5-fold.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Keyword
- EnterococcusPathway engineeringMevalonate
Publication and Content Type
- art (subject category)
- ref (subject category)
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