SwePub
Sök i LIBRIS databas

  Extended search

id:"swepub:oai:slubar.slu.se:122283"
 

Search: id:"swepub:oai:slubar.slu.se:122283" > Core genome multilo...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Core genome multilocus sequence typing (cgMLST) confirms systemic spread of avian pathogenic Escherichia coli (APEC) in broilers with cellulitis

Jonare, Liv (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
Hansson, Ingrid (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för biomedicin och veterinär folkhälsovetenskap (BVF),Department of Biomedical Science and Veterinary Public Health
Jansson, Desirée (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,National Veterinary Institute (SVA)
 (creator_code:org_t)
 
2023
2023
English.
In: Veterinary Microbiology. - 0378-1135 .- 1873-2542. ; 282
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • Broiler cellulitis has emerged as an important cause of economic losses for farmers and slaughter plants from carcass condemnation at processing. Avian pathogenic Escherichia coli (APEC) has been identified as the main causative agent. The aim was to characterize E. coli isolated from cellulitis and organs in broilers at slaughter by whole genome sequencing analysis to study if systemic spread could be confirmed. Isolates were collected postmortem from 101 carcasses condemned due to dermatitis/cellulitis from five commercial farms and six flocks. Forty-six isolates were characterised to determine serotypes, sequence types and virulence-associated genes. Analysis by cgMLST was performed to study the genetic similarity between isolates from the same broiler, among birds from the same flock and between flocks. Escherichia coli was isolated from 90% of birds from subcutaneous samples. In 20 broilers, E. coli was isolated from organs in pure culture or mixed with sparse growth of other bacteria. In eight of these, there were post-mortem findings suggestive of systemic bacterial spread. The majority of the isolates from the same bird and flock belonged to the same serotype and sequence type and were genetically indistinguishable, but differed when compared between flocks. Common APEC virulence genes, i.e. chuA, fyuA, hlyF, iroN, irp2, iss, ompT, sitA, TerC, TraT, were present in > 87% of the isolates. We conclude that evidence of systemic spread of E. coli from cellulitis was present in some birds at time of slaughter but cannot be reliably detected at meat inspection.

Subject headings

LANTBRUKSVETENSKAPER  -- Veterinärmedicin -- Patobiologi (hsv//swe)
AGRICULTURAL SCIENCES  -- Veterinary Science -- Pathobiology (hsv//eng)
LANTBRUKSVETENSKAPER  -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
AGRICULTURAL SCIENCES  -- Veterinary Science -- Clinical Science (hsv//eng)

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Find more in SwePub

By the author/editor
Jonare, Liv
Hansson, Ingrid
Jansson, Desirée
About the subject
AGRICULTURAL SCIENCES
AGRICULTURAL SCI ...
and Veterinary Scien ...
and Pathobiology
AGRICULTURAL SCIENCES
AGRICULTURAL SCI ...
and Veterinary Scien ...
and Clinical Science
Articles in the publication
Veterinary Micro ...
By the university
Swedish University of Agricultural Sciences

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view