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Ratiometric tempera...
Ratiometric temperature sensing with semiconducting polymer dots
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- Ye, Fangmao (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Wu, Changfeng (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Jin, Yuhui (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Chan, Yang-Hsiang (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Zhang, Xuanjun (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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- Chiu, Daniel T (författare)
- Department of Chemistry, University of Washington, Seattle, USA
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(creator_code:org_t)
- 2011-05-11
- 2011
- Engelska.
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 133:21, s. 8146-8149
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye—Rhodamine B (RhB), whose emission intensity decreases with increasing temperature—within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
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- art (ämneskategori)
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