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Single mimivirus pa...
Single mimivirus particles intercepted and imaged with an X-ray laser
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- Seibert, M. Marvin (author)
- Uppsala universitet,Molekylär biofysik
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- Ekeberg, Tomas (author)
- Uppsala universitet,Molekylär biofysik
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- Maia, Filipe R. N. C. (author)
- Uppsala universitet,Molekylär biofysik
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- Svenda, Martin (author)
- Uppsala universitet,Molekylär biofysik
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- Andreasson, Jakob (author)
- Uppsala universitet,Molekylär biofysik
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- Joensson, Olof (author)
- Uppsala universitet,Molekylär biofysik
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- Odic, Dusko (author)
- Uppsala universitet,Molekylär biofysik
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- Iwan, Bianca (author)
- Uppsala universitet,Molekylär biofysik
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- Rocker, Andrea (author)
- Uppsala universitet,Molekylär biofysik
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- Westphal, Daniel (author)
- Uppsala universitet,Molekylär biofysik
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- Hantke, Max (author)
- Uppsala universitet,Molekylär biofysik
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DePonte, Daniel P. (author)
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Barty, Anton (author)
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Schulz, Joachim (author)
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Gumprecht, Lars (author)
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Coppola, Nicola (author)
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Aquila, Andrew (author)
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Liang, Mengning (author)
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White, Thomas A. (author)
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Martin, Andrew (author)
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- Caleman, Carl (author)
- Uppsala universitet,Molekylär biofysik
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Stern, Stephan (author)
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Abergel, Chantal (author)
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Seltzer, Virginie (author)
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Claverie, Jean-Michel (author)
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Bostedt, Christoph (author)
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Bozek, John D. (author)
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Boutet, Sebastien (author)
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Miahnahri, A. Alan (author)
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Messerschmidt, Marc (author)
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Krzywinski, Jacek (author)
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Williams, Garth (author)
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Hodgson, Keith O. (author)
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Bogan, Michael J. (author)
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Hampton, Christina Y. (author)
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Sierra, Raymond G. (author)
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Starodub, Dmitri (author)
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- Andersson, Inger (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för molekylärbiologi,Department of Molecular Biology
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Bajt, Sasa (author)
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Barthelmess, Miriam (author)
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Spence, John C. H. (author)
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Fromme, Petra (author)
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Weierstall, Uwe (author)
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Kirian, Richard (author)
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Hunter, Mark (author)
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Doak, R. Bruce (author)
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Marchesini, Stefano (author)
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Hau-Riege, Stefan P. (author)
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Frank, Matthias (author)
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Shoeman, Robert L. (author)
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Lomb, Lukas (author)
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Epp, Sascha W. (author)
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Hartmann, Robert (author)
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Rolles, Daniel (author)
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Rudenko, Artem (author)
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Schmidt, Carlo (author)
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Foucar, Lutz (author)
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Kimmel, Nils (author)
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Holl, Peter (author)
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Rudek, Benedikt (author)
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Erk, Benjamin (author)
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Hoemke, Andre (author)
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Reich, Christian (author)
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Pietschner, Daniel (author)
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Weidenspointner, Georg (author)
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Strueder, Lothar (author)
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Hauser, Guenter (author)
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Gorke, Hubert (author)
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Ullrich, Joachim (author)
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Schlichting, Ilme (author)
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Herrmann, Sven (author)
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Schaller, Gerhard (author)
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Schopper, Florian (author)
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Soltau, Heike (author)
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Kuehnel, Kai-Uwe (author)
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Andritschke, Robert (author)
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Schroeter, Claus-Dieter (author)
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Krasniqi, Faton (author)
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Bott, Mario (author)
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Schorb, Sebastian (author)
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Rupp, Daniela (author)
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Adolph, Marcus (author)
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Gorkhover, Tais (author)
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Hirsemann, Helmut (author)
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Potdevin, Guillaume (author)
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Graafsma, Heinz (author)
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Nilsson, Björn (author)
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Chapman, Henry N. (author)
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- Hajdu, Janos (author)
- Uppsala universitet,Molekylär biofysik
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(creator_code:org_t)
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- 2011-02-02
- 2011
- English.
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 470:7332, s. 78-81
- Related links:
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https://bib-pubdb1.d...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Subject headings
Close
- X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions(1-4). Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma(1). The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval(2). Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a noncrystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source(5). Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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Nature
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Seibert, M. Marv ...
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Ekeberg, Tomas
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Maia, Filipe R. ...
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Svenda, Martin
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Andreasson, Jako ...
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Joensson, Olof
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show more...
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Odic, Dusko
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Iwan, Bianca
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Rocker, Andrea
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Westphal, Daniel
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Hantke, Max
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DePonte, Daniel ...
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Barty, Anton
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Schulz, Joachim
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Gumprecht, Lars
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Coppola, Nicola
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Aquila, Andrew
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Liang, Mengning
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White, Thomas A.
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Martin, Andrew
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Caleman, Carl
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Stern, Stephan
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Abergel, Chantal
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Seltzer, Virgini ...
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Claverie, Jean-M ...
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Bostedt, Christo ...
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Bozek, John D.
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Boutet, Sebastie ...
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Miahnahri, A. Al ...
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Messerschmidt, M ...
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Krzywinski, Jace ...
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Williams, Garth
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Hodgson, Keith O ...
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Bogan, Michael J ...
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Hampton, Christi ...
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Sierra, Raymond ...
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Starodub, Dmitri
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Andersson, Inger
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Bajt, Sasa
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Barthelmess, Mir ...
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Spence, John C. ...
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Fromme, Petra
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Weierstall, Uwe
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Kirian, Richard
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Hunter, Mark
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Doak, R. Bruce
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Marchesini, Stef ...
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Hau-Riege, Stefa ...
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Frank, Matthias
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Shoeman, Robert ...
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Lomb, Lukas
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Epp, Sascha W.
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Hartmann, Robert
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Rolles, Daniel
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Rudenko, Artem
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Schmidt, Carlo
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Foucar, Lutz
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Kimmel, Nils
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Holl, Peter
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Rudek, Benedikt
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Erk, Benjamin
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Hoemke, Andre
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Reich, Christian
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Pietschner, Dani ...
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Weidenspointner, ...
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Strueder, Lothar
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Hauser, Guenter
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Gorke, Hubert
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Ullrich, Joachim
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Schlichting, Ilm ...
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Herrmann, Sven
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Schaller, Gerhar ...
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Schopper, Floria ...
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Soltau, Heike
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Kuehnel, Kai-Uwe
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Andritschke, Rob ...
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Schroeter, Claus ...
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Krasniqi, Faton
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Bott, Mario
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Schorb, Sebastia ...
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Rupp, Daniela
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Adolph, Marcus
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Gorkhover, Tais
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Hirsemann, Helmu ...
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Potdevin, Guilla ...
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Graafsma, Heinz
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Nilsson, Björn
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Chapman, Henry N ...
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Hajdu, Janos
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Nature
- By the university
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Uppsala University
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Swedish University of Agricultural Sciences