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Blood group A glyco...
Blood group A glycolipid antigen biosynthesis: discrimination between biosynthesized and enzyme preparation derived blood group A antigen by mass spectrometry.
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Holgersson, J (författare)
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Jacobsson, A (författare)
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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visa fler...
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Samuelsson, B E (författare)
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visa färre...
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(creator_code:org_t)
- 1990
- 1990
- Engelska.
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Ingår i: Analytical biochemistry. - 0003-2697. ; 184:1, s. 145-50
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- A monofucosyl type 1 chain blood group A hexaglycosylceramide was biosynthesized in solution using the type 1 chain blood group H pentaglycosylceramide as precursor, a crude microsomal fraction prepared from the mucosa scraping of a blood group A pig small intestine as enzyme source, and uridine diphosphate-N-acetyl-(1-14C)galactosamine as sugar donor. The radioactive product was enriched using reversed-phase column chromatography and silica gel HPLC. The peak, as detected by a beta-flow scintillation counter, was collected, permethylated, and analyzed by mass spectrometry. Carbohydrate sequence ions were found, indicating the presence of both the biosynthesized and a native, non-14C-containing blood group A hexaglycosylceramide. The blood group A pig small intestinal mucosa used as the enzyme source contain blood group A hexaglycosylceramide as the predominant glycolipid. Therefore, it is concluded that the nonradioactive blood group A hexaglycosylceramide found after the biosynthesis is derived from the enzyme preparation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- ABO Blood-Group System
- Animals
- Autoradiography
- Chromatography
- High Pressure Liquid
- Chromatography
- Thin Layer
- Glycolipids
- isolation & purification
- Mass Spectrometry
- Methylation
- Silica Gel
- Silicon Dioxide
- Swine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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