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Nuclear import of g...
Nuclear import of glucokinase in pancreatic beta-cells is mediated by a nuclear localization signal and modulated by SUMOylation
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- Johansson, Bente Berg (författare)
- University of Bergen,Haukeland University Hospital
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- Fjeld, Karianne (författare)
- Haukeland University Hospital,University of Bergen
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- Solheim, Marie Holm (författare)
- Haukeland University Hospital,University of Bergen,Harvard University
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- Shirakawa, Jun (författare)
- Harvard University,Yokohama City University
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- Zhang, Enming (författare)
- Lund University,Lunds universitet,Diabetes - öpatofysiologi,Forskargrupper vid Lunds universitet,Diabetes - Islet Patophysiology,Lund University Research Groups
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- Keindl, Magdalena (författare)
- University of Bergen,Haukeland University Hospital
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- Hu, Jiang (författare)
- Harvard University
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- Lindqvist, Andreas (författare)
- Lund University,Lunds universitet,Neuroendokrin cellbiologi,Forskargrupper vid Lunds universitet,Neuroendocrine Cell Biology,Lund University Research Groups
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- Døskeland, Anne (författare)
- University of Bergen
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- Mellgren, Gunnar (författare)
- University of Bergen,Haukeland University Hospital
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- Flatmark, Torgeir (författare)
- University of Bergen
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- Njølstad, Pål Rasmus (författare)
- Haukeland University Hospital,University of Bergen
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- Kulkarni, Rohit N. (författare)
- Harvard University
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- Wierup, Nils (författare)
- Lund University,Lunds universitet,Neuroendokrin cellbiologi,Forskargrupper vid Lunds universitet,Neuroendocrine Cell Biology,Lund University Research Groups
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- Aukrust, Ingvild (författare)
- University of Bergen
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- Bjørkhaug, Lise (författare)
- Western Norway University of Applied Sciences,University of Bergen
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- Engelska.
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Ingår i: Molecular and Cellular Endocrinology. - : Elsevier BV. - 0303-7207. ; 454, s. 146-157
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The localization of glucokinase in pancreatic beta-cell nuclei is a controversial issue. Although previous reports suggest such a localization, the mechanism for its import has so far not been identified. Using immunofluorescence, subcellular fractionation and mass spectrometry, we present evidence in support of glucokinase localization in beta-cell nuclei of human and mouse pancreatic sections, as well as in human and mouse isolated islets, and murine MIN6 cells. We have identified a conserved, seven-residue nuclear localization signal (30LKKVMRR36) in the human enzyme. Substituting the residues KK31,32 and RR35,36 with AA led to a loss of its nuclear localization in transfected cells. Furthermore, our data indicates that SUMOylation of glucokinase modulates its nuclear import, while high glucose concentrations do not significantly alter the enzyme nuclear/cytosolic ratio. Thus, for the first time, we provide data in support of a nuclear import of glucokinase mediated by a redundant mechanism, involving a nuclear localization signal, and which is modulated by its SUMOylation. These findings add new knowledge to the functional role of glucokinase in the pancreatic beta-cell.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Beta-cells
- Glucokinase
- Human islets
- MIN6 cells
- Nuclear localization signal
- Pancreatic islets
- SUMOylation
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- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Johansson, Bente ...
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Fjeld, Karianne
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Solheim, Marie H ...
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Shirakawa, Jun
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Zhang, Enming
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Keindl, Magdalen ...
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visa fler...
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Hu, Jiang
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Lindqvist, Andre ...
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Døskeland, Anne
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Mellgren, Gunnar
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Flatmark, Torgei ...
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Njølstad, Pål Ra ...
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Kulkarni, Rohit ...
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Wierup, Nils
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Aukrust, Ingvild
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Bjørkhaug, Lise
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visa färre...
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