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Flow cytometric analysis of myelodysplasia : Pre-analytical and technical issues—Recommendations from the European LeukemiaNet

van der Velden, Vincent H.J. (author)
Erasmus University Medical Center
Preijers, Frank (author)
Radboud University Medical Center
Johansson, Ulrika (author)
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Westers, Theresia M. (author)
Amsterdam UMC - Vrije Universiteit Amsterdam
Dunlop, Alan (author)
Royal Marsden Hospital, London
Porwit, Anna (author)
Lund University,Lunds universitet,Patologi, Lund,Sektion V,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Pathology, Lund,Section V,Department of Clinical Sciences, Lund,Faculty of Medicine
Béné, Marie C. (author)
Nantes University Hospital
Valent, Peter (author)
Medical University of Vienna
te Marvelde, Jeroen (author)
Erasmus University Medical Center
Wagner-Ballon, Orianne (author)
Henri Mondor Hospital
Oelschlaegel, Uta (author)
Saft, Leonie (author)
Karolinska Institutet,Karolinska University Hospital
Kordasti, Sharham (author)
Guy's Hospital
Ireland, Robin (author)
Guy's Hospital
Cremers, Eline (author)
Yukioka Hospital
Alhan, Canan (author)
Amsterdam UMC - Vrije Universiteit Amsterdam
Duetz, Carolien (author)
Amsterdam UMC - Vrije Universiteit Amsterdam
Hobo, Willemijn (author)
Radboud University Medical Center
Chapuis, Nicolas (author)
Assistance Publique des Hôpitaux de Paris
Fontenay, Michaela (author)
Assistance Publique des Hôpitaux de Paris
Bettelheim, Peter (author)
Eidenshink-Brodersen, Lisa (author)
Hematologics Inc.
Font, Patricia (author)
Hospital Gregorio Maranon
Loken, Michael R. (author)
Hematologics Inc.
Matarraz, Sergio (author)
University of Salamanca,Carlos III Health Institute
Ogata, Kiyoyuki (author)
Orfao, Alberto (author)
University of Salamanca,Carlos III Health Institute
Psarra, Katherina (author)
Evangelismos Athens General Hospital
Subirá, Dolores (author)
Hospital Universitario de Guadalajara
Wells, Denise A. (author)
Hematologics Inc.
Della Porta, Matteo G. (author)
Humanitas University
Burbury, Kate (author)
University of Melbourne
Bellos, Frauke (author)
Munich Leukemia Laboratory (MLL)
Weiß, Elisabeth (author)
Munich Leukemia Laboratory (MLL)
Kern, Wolfgang (author)
Munich Leukemia Laboratory (MLL)
van de Loosdrecht, Arjan (author)
Amsterdam UMC - Vrije Universiteit Amsterdam
Weiss, E (author)
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 (creator_code:org_t)
2021-12-11
2023
English.
In: Cytometry Part B - Clinical Cytometry. - : Wiley. - 1552-4949 .- 1552-4957. ; 104:1, s. 15-26
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. Conclusions: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Hematologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Hematology (hsv//eng)

Keyword

ELN
flow cytometry
MDS
pre-analytic issues

Publication and Content Type

art (subject category)
ref (subject category)

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