| 131. |
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| 132. |
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| 133. |
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| 134. |
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| 135. |
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| 136. |
- Belfrage, Per, et al.
(författare)
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Alterations of lipid metabolism in healthy volunteers during long-term ethanol intake
- 1977
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Ingår i: European Journal of Clinical Investigation. - : Wiley. - 0014-2972 .- 1365-2362. ; 7:2, s. 127-131
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Tidskriftsartikel (refereegranskat)abstract
- Nine young, healthy male volunteers were given ethanol (75 g/day) for 5 weeks. The ethanol was divided into five daily doses and taken so that blood ethanol levels never exceeded 0.04% (w/v). During the latter part of the ethanol intake period, there was a significant, transient increase of plasma triglyceride (TG) concentrations followed by reduction to normal levels. A three-fold increase of lipoprotein lipase activity (LLA) occurred in biopsy specimens of adipose tissue. An increase of alpha-lipoprotein concentrations, which correlated significantly with the decrease in plasma TG levels and the increase in adipose LLA, was also observed during the ethanol intake period. No changes were observed in plasma cholesterol and beta-lipoprotein levels. A transient, three-fold increase of TG concentrations occurred in liver biopsy specimens. Ultrastructural and cytochemical examinations of the biopsy specimens showed hyperplasia of the smooth endoplasmic reticulum, and increased canallicular activity of gamma-glutamyl transferase (gamma-GT) activity in most subjects towards the end of and after the ethanol intake period. Serum gamma-GT levels also increased significantly.
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| 137. |
- Belfrage, Per, et al.
(författare)
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Dispersion of viable pig liver cells with collagenase
- 1975
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Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
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Tidskriftsartikel (refereegranskat)abstract
- Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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| 138. |
- Belfrage, Per, et al.
(författare)
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Methods for the determination in the nanomole range of lipids in liver fine-needle aspiration biopsies
- 1970
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Ingår i: Scandinavian Journal of Clinical & Laboratory Investigation. - : Informa UK Limited. - 1502-7686 .- 0036-5513. ; 26:1, s. 53-60
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Tidskriftsartikel (refereegranskat)abstract
- Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.
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| 139. |
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| 140. |
- Belin, G, et al.
(författare)
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Electronic gJ Factors, Natural Lifetimes, and Electric Quadrupole Interaction for Rb 87 in the np ^(2)P_(3/2) Series of the Rb I Spectrum
- 1971
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Ingår i: Physica Scripta. - : IOP Publishing. - 0031-8949 .- 1402-4896. ; 4:6, s. 269-273
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Tidskriftsartikel (refereegranskat)abstract
- For Rb 87 the level crossing method was applied to the first four levels ( n = 5, 6, 7, and 8) of the np 2 P 3/2 series in the Rb I spectrum. By using magnetic dipole interaction constants from corresponding zero field optical double resonance experiments, the electronic g J factors of 5, 6, and 7 p 2 P 3/2 were calculated from the level crossing data. For these levels the natural radiative lifetimes were determined from the half-widths of the level crossing signals. For 8 p 2 P 3/2 the a factor was obtained assuming g J = -1.336 (2). From the measured electric quadrupole interaction constants b , decreasing values of the nuclear quadrupole moment Q were obtained in 5, 6, 7, and 8 p 2 P 3/2 . When core polarization effects are taken into account, the different 2 P 3/2 levels yield consistent values for the quadrupole moment. We find ----- -- g J (5 p 2 P 3/2 ) = -1.3362 (6) -- g J (6 p 2 P 3/2 ) = -1.3347 (10) ----- -- Ï (5 p 2 P 3/2 ) = 25.8 (8) ns -- Ï (6 p 2 P 3/2 ) = 111 (3) ns ----- -- b (5 p 2 P 3/2 ) = 12.510 (57) MHz -- b (6 p 2 P 3/2 ) = 3.947 (13) MHz ----- -- Q (5 p 2 P 3/2 ) = 0.131 barn -- Q (6 p 2 P 3/2 ) = 0.133 barn ----- -- g J (7 p 2 P 3/2 ) = -1.3359 (11) -- a (8 p 2 P 3/2 ) = 6.747 (14) MHz ----- -- Ï (7 p 2 P 3/2 ) = 233 (10) ns -- b (8 p 2 P 3/2 ) = 0.933 (20) MHz ----- -- b (7 p 2 P 3/2 ) = 1.768 (8) MHz -- Q (8 p 2 P 3/2 ) = 0.131 barn ----- -- Q (7 p 2 P 3/2 ) = 0.133 barn -- ----- Considering possible sources of error in the evaluation of the quadrupole moment we obtain Q (Rb 87 ) = +0.132 (9) barn.
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