| 11. |
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| 12. |
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| 13. |
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| 14. |
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| 15. |
- Degerman, Eva, et al.
(författare)
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Single-step affinity purification, partial structure and properties of human platelet cGMP inhibited cAMP phosphodiesterase
- 1994
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002. ; 1205:2, s. 189-198
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Tidskriftsartikel (refereegranskat)abstract
- The human platelet cilostamide- and cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) was rapidly purified approximately 19,000-fold to apparent homogeneity using single step affinity chromatography on the isothiocyanate derivative of cilostamide coupled to aminoethyl agarose. Within 24 h, 30 micrograms of enzyme protein was obtained from 20 ml of packed platelets. Vmax for cAMP and cGMP was 6.1 and 0.9 mumol/min per mg protein, respectively. Several polypeptides (110/105, 79, 62, 55/53 kDa) were identified after SDS-PAGE, all of which were immunologically related to cGI-PDE and represented approx. 5, 20, 50 and 20% of the total protein, respectively. Limited proteolysis of the cGI-PDE with chymotrypsin produced a major fragment of approximately 47 kDa (and at least two smaller peptides) with catalytic activity and sensitivity to cGMP and OPC 3911 similar to controls. Phosphorylation of the cGI-PDE by cAMP-dependent protein kinase (A-kinase) resulted in maximal incorporation of 0.6-1.8 mol of 32P/mol 110/105 and 79 kDa polypeptides; much lower and variable amounts of phosphate were incorporated into the 62 and 55/53 kDa polypeptides. After digestion of cGI-PDE with several proteinases a number of peptides were isolated and sequenced. Most of the peptide sequences obtained could be aligned within the carboxy terminal domain of the deduced sequence of the human cardiac cGI-PDE. These and other results suggest that the subunit size of the intact platelet cGI-PDE is 110 kDa and that proteolytic fragments of 79, 62 and 55/53 kDa are produced during purification. The smaller fragments (62 and 55/53 kDa) contain the catalytic domain; the larger fragments (110 and 79 kDa) also contain the regulatory domain with phosphorylation sites for A-kinase.
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| 16. |
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| 17. |
- Dyrehag, L E, et al.
(författare)
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Effects of repeated sensory stimulation sessions (electro-acupuncture) on skin temperature in chronic pain patients.
- 1997
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Ingår i: Scandinavian journal of rehabilitation medicine. - 0036-5505. ; 29:4, s. 243-50
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Tidskriftsartikel (refereegranskat)abstract
- Changes in skin temperature and haemodynamics were studied during experimentally administered electro-acupuncture before and after a 4-week period of electro-acupuncture (EA) treatments. Subjective pain intensity was evaluated using a pain questionnaire. Twelve patients with long-lasting nociceptive pain were included. Before clinical treatment, skin temperature tended to decrease after 30 minutes' stimulation. In contrast, a significant increase was seen after the clinical treatment. No significant changes were seen for blood pressure, heart rate or pain intensity before and after the clinical treatment. The data indicate that an increased skin vasoconstrictor sympathetic activity may be responsible for the decreased skin temperature during the electro-acupuncture in the initial test sessions, whereas an inhibition of skin sympathetic activity and/or a release of vasodilatory substances may be responsible for the increase in temperature after completed clinical treatment. Despite a small number of subjects and correction for multiple inference, the difference in temperature effects before and up to 3 months after acupuncture treatment was significant.
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| 18. |
- Dyrehag, L E, et al.
(författare)
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Relations between self-rated musculoskeletal symptoms and signs and psychological distress in chronic neck and shoulder pain.
- 1998
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Ingår i: Scandinavian journal of rehabilitation medicine. - 0036-5505. ; 30:4, s. 235-42
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Tidskriftsartikel (refereegranskat)abstract
- The purposes of the present study were to describe physical and psychological characteristics of 55 chronic pain patients with predominantly nociceptive neck and shoulder complaints, and to explore relationships between physical assessment methods, self-reported pain and psychological distress. The physical measures included cervical and shoulder mobility and muscle tenderness. The Pain Severity and Interference subscales from the Multidimensional Pain Inventory (MPI), Becks Depression Inventory (BDI), State-Trait Anxiety Inventory (STAI-Y), and a pain drawing assessed self-reports of pain and psychological distress. The number of tender points (TP score) correlated significantly with pain severity, (p < 0.01) Interference (p < 0.05), pain drawing score (p < 0.05), BDI (p < 0.05) and state anxiety (p < 0.05). No significant correlation was seen between TP score and age, pain duration or trait anxiety. The results suggest that there are relationships between observers' ratings of muscle tenderness (TP score) and self-reports of pain severity, interference of pain and psychological distress in patients with chronic cervico-brachial pain.
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