31. |
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32. |
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33. |
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34. |
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35. |
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36. |
- Antuch, W, et al.
(författare)
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The NMR solution structure of a Kunitz-type proteinase inhibitor from the sea anemone Stichodactyla helianthus.
- 1993
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Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 212, s. 675-684
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Tidskriftsartikel (refereegranskat)abstract
- The solution structure of a 55-amino-acid Kunitz-type proteinase inhibitor, ShPI, purified from the Caribbean sea anemone Stichodactyla helianthus, was determined by NMR spectroscopy. Nearly complete sequence-specific 1H-NMR assignments were obtained at pH 4.6 and 36 degrees C, and stereo-specific assignments were determined for 23 pairs of diastereotopic substituents. A data set of 666 upper distance limit constraints and 122 dihedral angle constraints collected on this basis was used as input for a structure calculation with the program DIANA. Following energy minimization with the program OPAL, the average root-mean-square diviation (RMSD) of the 20 DIANA conformers used to represent the solution structure relative to the mean structure is 61 pm for all backbone atoms N, C alpha and C', and 106 pm for all heavy atoms of residues 2-53. This high-quality solution structure of ShPI has a nearly identical molecular architecture as the bovine pancreatic trypsin inhibitor (BPTI), despite a mere 35% of sequence similarity between the two proteins. Exchange rates measured for 48 out of the 51 backbone amide protons showed that the positions of 20 slowly exchanging amide protons correlate well with hydrogen bonds involving these protons in the energy-minimized solution structure. The solution structure of ShPI is compared to the four homologous proteins for which the three-dimensional structure is also available.
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37. |
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Arkitekturteorier
- 1999
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)
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38. |
- Arrhenius, Sara, et al.
(författare)
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Ensam och pervers
- 1995
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Bok (populärvet., debatt m.m.)
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39. |
- Arup, Ulf, et al.
(författare)
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High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances.
- 1993
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Ingår i: The Lichenologist. - : Cambridge University Press (CUP). - 0024-2829 .- 1096-1135. ; 25, s. 61-71
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Tidskriftsartikel (refereegranskat)abstract
- High performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in accordance with the standardized TLC method. A revised table for the identification of 69 lichen substances (obtained from 62 taxa) is accordingly presented.
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40. |
- Avatare, Anneli, et al.
(författare)
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The APS/AMID Project
- 1991
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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