| 41. |
- Denisov, Vladimir P., et al.
(författare)
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Kinetics of DNA hydration
- 1997
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836. ; 268:1, s. 118-136
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Tidskriftsartikel (refereegranskat)abstract
- The hydration of the d(CGCGAATTCGCG) B-DNA duplex in solution was studied by nuclear magnetic relaxation dispersion (NMRD) of the water nuclei 1H, 2H, and 17O, and by nuclear Overhauser effects (NOEs) in high-resolution two-dimensional 1H NMR spectra. By comparing results from the free duplex with those from its complex with netropsin, water molecules in the 'spine of hydration' in the AATT region of the minor groove could be distinguished from hydration water elsewhere in the duplex. The 2H and 17O relaxation dispersions yield a model-independent residence time of 0.9(±0.1) ns at 4°C for five highly ordered water molecules in the spine. When corrected for frequency offset effects, the NOE data yield the same residence time as the NMRD data, giving credence to both methods. At 27°C, the residence time is estimated to 0.2 ns, a factor of 40 shorter than the tumbling time of the duplex. The NMRD data show that all water molecules associated with the duplex, except the five molecules in the spine, have residence times significantly shorter than Ins at 4°C. There is thus no long-lived hydration structure associated with the phosphate backbone. In contrast to 2H and 17O, the 1H relaxation dispersion is dominated by labile DNA protons and therefore provides little information about DNA hydration.
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| 42. |
- Dincbas, V, et al.
(författare)
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Shutdown in protein synthesis due to the expression of mini-genes in bacteria
- 1999
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS LTD. - 0022-2836. ; 291:4, s. 745-759
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Mutants of Escherichia coli partially deficient in peptidyl-tRNA hydrolase are killed by the expression of certain very short open reading frames (mini-genes), encoded by the wild-type bar regions of phage lambda. According to the current hypothesis, prot
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| 43. |
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| 44. |
- Dong, HJ, et al.
(författare)
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Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates
- 1996
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS LTD. - 0022-2836. ; 260:5, s. 649-663
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We have used two-dimensional polyacrylamide gel electrophoresis to fractionate tRNAs from Escherichia coli. A sufficiently high degree of resolution was obtained for 44 out of 46 tRNA species in E, coli to be resolved into individual electrophoretic compo
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| 45. |
- Dong, HJ, et al.
(författare)
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Growth rate regulation of 4.5 S RNA and M1 RNA the catalytic subunit of Escherichia coli RNase P
- 1996
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS LTD. - 0022-2836. ; 261:3, s. 303-308
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We have studied the expression of 4.5 S RNA and Fv Il RNA, the catalytic subunit of Escherchia coli RNase P, under various growth conditions. Both RNA species increase in abundance as a function of growth rate. There are roughly 450 molecules of 4.5 S RNA
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| 46. |
- DONG, HJ, et al.
(författare)
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RIBOSOME MUTANTS WITH ALTERED ACCURACY TRANSLATE WITH REDUCED PROCESSIVITY
- 1995
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS (LONDON) LTD. - 0022-2836. ; 248:3, s. 551-561
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We have determined whether or not there is a correlation between the accuracy of tRNA selection and the processivity of translation by mutant ribosomes. In our assay the estimation of processivity was made from a system in which transcription is dependent
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| 47. |
- EHRENBERG, M, et al.
(författare)
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A MODEL FOR COPY NUMBER CONTROL OF THE PLASMID R1
- 1995
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS (LONDON) LTD. - 0022-2836. ; 246:4, s. 472-485
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- A new model for copy numb er control of the plasmid R1 has been develop ed. It takes into account that initiation of replication of R1 requires a large number of cis-acting proteins (RepA). The theory explains how plasmid production rates respond to shift
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| 48. |
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| 49. |
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| 50. |
- FLOCCO, MM, et al.
(författare)
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STRANGE BEDFELLOWS - INTERACTIONS BETWEEN ACIDIC SIDE-CHAINS IN PROTEINS
- 1995
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - : ACADEMIC PRESS (LONDON) LTD. - 0022-2836. ; 254:1, s. 96-105
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The oxygen atoms of two acidic side-chains are frequently found within hydrogen-bonding distance of each other in proteins. Two distinct types of cases are common. In metal-binding sites, the oxygen atoms are brought near (average closest approach 3.0 Ang
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